RNA DIRECTED DNA POLYMERASE & 70S RNA OF ONCORNAVIRUSES

在此更新补助金申请中提出的研究 继续我们正在进行的逆转录病毒性质的研究， 先前声称存在于细胞类型中的相关结构， 缺乏内源性逆转录病毒。 我们最近已经证明， 几种以前被认为缺乏鸟类细胞的鸟类细胞， 逆转录病毒相关序列实际上在逆转录病毒载体中含有这样的序列。 两种基因结构的形式。 一组的结构 病毒相关的遗传序列作为内源性逆转录病毒存在， 因为它们含有gag-、pol-、env和LTR相关序列 以原病毒的形式相互连接 的结构 另一组逆转录病毒相关的基因序列单独存在 gag-、pol-、env-和LTR相关序列不与一个 另一个是附条款 为了阐明 这些新的禽类内源性逆转录病毒家族的性质 而单独的逆转录病毒相关基因序列呈现细胞 类型，我们计划：1）隔离和表征这两类 存在于这些细胞类型中的禽逆转录病毒基因序列， （2）确定其确切性质;（3）确定 描述其他鸟类中的转录相关结构 物种以及其他脊椎动物属，试图定义 更远距离进化的序列和新类别的性质 内源性逆转录病毒 我们计划采用的方法 为实现这些目标，在很大程度上， 在我们的实验室中实现，包括各种 理化、酶学和杂交技术 可用于DNA、RNA和蛋白质的分析，

The studies proposed in this renewal grant application exemplify a continuation of our ongoing work on the nature of retrovirus- related structures present in cell types previously purported to lack endogenous retroviruses. We have recently demonstrated that several species of avian cells previously thought to lack avian retrovirus-related sequences in fact contain such sequences in the form of two genetic structures. The structure of one group of viral-related genetic sequences exists as endogenous retroviruses, since they contain gag-, pol-, env and LTR-related sequences linked to one another in the form of a provirus. The structure of the other group of retrovirus-related genetic sequences exists as solo gag-, pol-, env-, and LTR-related sequences not linked to one another in the form of a provisus. In an effort to shed light on the nature of these novel families of avian endogenous retroviruses and the solo retrovirus-related genetic sequences present cell types, we plan to: 1) isolate and characterize both classes of avian retrovirus genetic sequences present in these cell types in order to delineate their precise nature; 2) identify and characterize the transcriptional related structures in other avian species as well as other vertebrate genera in an attempt to define the nature of more distantly-evolved sequences and novel classes of endogenous retroviruses. The methodologies that we plan to employ to achieve these objectives are, for the most part, already implemented in our laboratory and include a variety of physicochemical, enzymological, and hybridization techniques available for the analysis of DNA, RNA, and protein,