RNA DIRECTED DNA POLYMERASE & 70S RNA OF ONCORNAVIRUSES

RNA定向DNA聚合酶

• 批准号: 3164916
• 负责人: ANTHONY J FARAS
• 金额: $ 12.07万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-06-01 至 1994-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3164916
• 关键词: Aves; RNA directed DNA polymerase; Retroviridae; Retroviridae disease; alpharetrovirus; developmental genetics; gene expression; genetic library; genetic manipulation; genetic mapping; genetic transcription; laboratory rabbit; molecular cloning; molecular oncology; nucleic acid hybridization; nucleic acid sequence; oncogenic virus; radiotracer; temperature sensitive mutant; virus DNA; virus RNA; virus genetics; virus replication

The studies proposed in this renewal grant application exemplify a continuation of our ongoing work on the nature of retrovirus- related structures present in cell types previously purported to lack endogenous retroviruses. We have recently demonstrated that several species of avian cells previously thought to lack avian retrovirus-related sequences in fact contain such sequences in the form of two genetic structures. The structure of one group of viral-related genetic sequences exists as endogenous retroviruses, since they contain gag-, pol-, env and LTR-related sequences linked to one another in the form of a provirus. The structure of the other group of retrovirus-related genetic sequences exists as solo gag-, pol-, env-, and LTR-related sequences not linked to one another in the form of a provisus. In an effort to shed light on the nature of these novel families of avian endogenous retroviruses and the solo retrovirus-related genetic sequences present cell types, we plan to: 1) isolate and characterize both classes of avian retrovirus genetic sequences present in these cell types in order to delineate their precise nature; 2) identify and characterize the transcriptional related structures in other avian species as well as other vertebrate genera in an attempt to define the nature of more distantly-evolved sequences and novel classes of endogenous retroviruses. The methodologies that we plan to employ to achieve these objectives are, for the most part, already implemented in our laboratory and include a variety of physicochemical, enzymological, and hybridization techniques available for the analysis of DNA, RNA, and protein,

在这项续期拨款申请中提出的研究举例说明 我们正在进行的关于逆转录病毒性质的工作的继续- 出现在先前声称的细胞类型中的相关结构 缺乏内源性逆转录病毒。我们最近证明了 几种以前被认为缺乏禽类的鸟类细胞 逆转录病毒相关序列实际上包含这样的序列 两种遗传结构的形式。其中一组的结构 病毒相关基因序列以内源性逆转录病毒的形式存在， 因为它们含有与Gag-、Poll-、env和Ltr相关序列相连 以前病毒的形式相互传递。的结构。 另一组与逆转录病毒相关的基因序列作为单独存在 与Gag-、Poll-、Env-和Ltr相关的序列没有链接到一个 另一种是以但书的形式。为了让人们了解 这些新的禽内源性逆转录病毒家族的性质 与单反转录病毒相关的基因序列呈现细胞 类型，我们计划：1)分离和表征这两类 在这些细胞类型中存在的禽流感逆转录病毒基因序列 以描述它们的确切性质；2)识别和 其他禽类转录相关结构的特征 物种以及其他脊椎动物属，试图定义 更远进化的序列和新类的性质 内源性逆转录病毒。我们计划采用的方法 在很大程度上，实现这些目标的员工已经 在我们的实验室中实施，包括各种 物理化学、酶学和杂交技术 可用于DNA、RNA和蛋白质的分析，