RNA DIRECTED DNA POLYMERASE & 70S RNA OF ONCORNAVIRUSES

本次续展拨款申请中提出的研究举例说明 我们正在进行的关于逆转录病毒性质的工作的继续- 先前据称存在于细胞类型中的相关结构 缺乏内源性逆转录病毒。 我们最近证明了 以前认为几种鸟类细胞缺乏鸟类细胞 逆转录病毒相关序列实际上在 两种遗传结构的形式。 一组的结构 病毒相关的基因序列作为内源性逆转录病毒存在， 因为它们包含与 gag、pol、env 和 LTR 相关的序列 以原病毒的形式彼此结合。 的结构 另一组与逆转录病毒相关的基因序列单独存在 gag-、pol-、env- 和 LTR 相关序列不与任何一个连接 另一种是附带条款的形式。 为了阐明 这些禽类内源性逆转录病毒新家族的性质 以及单独的逆转录病毒相关基因序列呈现细胞 类型，我们计划：1）分离并表征这两类 这些细胞类型中存在的禽逆转录病毒基因序列 为了描述它们的精确性质； 2) 识别和 表征其他鸟类的转录相关结构 物种以及其他脊椎动物属试图定义 更遥远的进化序列和新类别的本质 内源性逆转录病毒。 我们计划采用的方法 在大多数情况下，为实现这些目标而雇用的人员已经 在我们的实验室实施，包括各种 物理化学、酶学和杂交技术 可用于 DNA、RNA 和蛋白质的分析，

The studies proposed in this renewal grant application exemplify a continuation of our ongoing work on the nature of retrovirus- related structures present in cell types previously purported to lack endogenous retroviruses. We have recently demonstrated that several species of avian cells previously thought to lack avian retrovirus-related sequences in fact contain such sequences in the form of two genetic structures. The structure of one group of viral-related genetic sequences exists as endogenous retroviruses, since they contain gag-, pol-, env and LTR-related sequences linked to one another in the form of a provirus. The structure of the other group of retrovirus-related genetic sequences exists as solo gag-, pol-, env-, and LTR-related sequences not linked to one another in the form of a provisus. In an effort to shed light on the nature of these novel families of avian endogenous retroviruses and the solo retrovirus-related genetic sequences present cell types, we plan to: 1) isolate and characterize both classes of avian retrovirus genetic sequences present in these cell types in order to delineate their precise nature; 2) identify and characterize the transcriptional related structures in other avian species as well as other vertebrate genera in an attempt to define the nature of more distantly-evolved sequences and novel classes of endogenous retroviruses. The methodologies that we plan to employ to achieve these objectives are, for the most part, already implemented in our laboratory and include a variety of physicochemical, enzymological, and hybridization techniques available for the analysis of DNA, RNA, and protein,