RAPID, OPTICAL NUCLEIC ACID PROBES FOR HIV DNA AND RNA

HIV DNA 和 RNA 的快速光学核酸探针

• 批准号: 2072211
• 负责人: ROBERT A LIEBERMAN
• 金额: $ 7.5万
• 依托单位: PHYSICAL OPTICS CORPORATION
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-05-01 至 1994-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2072211
• 关键词: biosensor device; charge coupled device camera; chemical binding; chemical bond resonance; fiber optics; human immunodeficiency virus; laser spectrometry; method development; nucleic acid hybridization; nucleic acid probes; virus DNA; virus RNA

DESCRIPTION (Adapted from applicant's abstract): The development of diverse nucleic probes is generating excitement because of their potential as diagnostic reagents. These tools promise to enhance the diagnosis of infectious diseases, genetic disorders, malignancies, and cancer. However, the extension of laboratory breakthroughs to routine clinical diagnostics has produced great disappointment. The reasons for the failure of the transition of these probes from the laboratory to the clinic are threefold: inadequate sensitivity, inadequate specificity, and inconvenience associated with the lack of user-friendly systems to monitor these probes. The proposal is to develop a multi-analyze nucleic acid probe system for the detection of HIV DNA and RNA that solves these problems using surface plasmon resonance (SPR). Because this detection technique is direct, without complex hybridization protocols, it promises to provide rapid screening of target analytes with minimal skill requirements. The benefit of the proposed system lies not solely in its sensitive detection of nucleic acid hybridization, but also in its ability to solve the problems plaguing SPR biosensors in general: these include inadequate specificity and inconvenience. Because of this, the proposed system also may significantly impact the immunodiagnostics community, where SPR sensing techniques have been under investigation for several years.

描述（改编自申请人摘要）： 不同的核酸探针正在产生兴奋，因为它们的 作为诊断试剂的潜力。 这些工具有望增强 传染病、遗传病、恶性肿瘤的诊断， 癌然而，实验室突破向常规的延伸 临床诊断已经产生了很大的失望。 的原因 这些探测器从实验室到实验室的过渡失败 临床上有三个方面：灵敏度不足，特异性不足， 以及与缺乏用户友好系统相关的不便， 监视这些探测器 建议开发一种多分析核酸 用于检测HIV DNA和RNA的酸性探针系统解决了这些问题， 表面等离子体共振（SPR）。 因为这种检测 技术是直接的，没有复杂的杂交方案， 有望提供目标分析物的快速筛选， 技能要求。 拟议系统的好处不仅在于 在其灵敏的核酸杂交检测中， 它能够解决SPR生物传感器普遍存在的问题： 这些问题包括不够具体和不方便。 因为 因此，拟议的系统也可能会显着影响 免疫诊断界，其中SPR传感技术已经 被调查了好几年。