RAPID, OPTICAL NUCLEIC ACID PROBES FOR HIV DNA AND RNA

描述(改编自申请人的摘要)：发展 多样化的核探测器引起了人们的兴奋，因为他们的 作为诊断试剂的潜力。这些工具承诺增强 诊断传染病、遗传性疾病、恶性肿瘤和 癌症。然而，实验室突破的延伸到常规 临床诊断结果令人大失所望。原因是 这些探头从实验室过渡到实验室的失败 临床表现有三个方面：敏感性不足，特异性不足， 以及与缺乏用户友好的系统相关的不便 监视这些探测器。这项提议是开发一种多分析的核酸 用于检测HIV DNA和RNA的酸性探针系统解决了这些问题 使用表面等离子激元共振(SPR)的问题。因为这次探测到 技术是直接的，没有复杂的杂交方案，它 承诺提供目标分析物的快速筛选，只需最少的 技能要求。拟议系统的好处不仅仅在于 在其灵敏的核酸杂交检测中，也在 它能够解决普遍困扰SPR生物传感器的问题： 这些问题包括不够具体和不方便。因为. 这一点，拟议的制度也可能显著影响 免疫诊断社区，SPR传感技术一直在那里 接受了几年的调查。

DESCRIPTION (Adapted from applicant's abstract): The development of diverse nucleic probes is generating excitement because of their potential as diagnostic reagents. These tools promise to enhance the diagnosis of infectious diseases, genetic disorders, malignancies, and cancer. However, the extension of laboratory breakthroughs to routine clinical diagnostics has produced great disappointment. The reasons for the failure of the transition of these probes from the laboratory to the clinic are threefold: inadequate sensitivity, inadequate specificity, and inconvenience associated with the lack of user-friendly systems to monitor these probes. The proposal is to develop a multi-analyze nucleic acid probe system for the detection of HIV DNA and RNA that solves these problems using surface plasmon resonance (SPR). Because this detection technique is direct, without complex hybridization protocols, it promises to provide rapid screening of target analytes with minimal skill requirements. The benefit of the proposed system lies not solely in its sensitive detection of nucleic acid hybridization, but also in its ability to solve the problems plaguing SPR biosensors in general: these include inadequate specificity and inconvenience. Because of this, the proposed system also may significantly impact the immunodiagnostics community, where SPR sensing techniques have been under investigation for several years.