SUBMICRON CHEMICAL SENSORS FOR INTRACELLULAR APPLICATION

适用于细胞内应用的亚微米化学传感器

• 批准号: 2189512
• 负责人: ROBERT A LIEBERMAN
• 金额: $ 32.31万
• 依托单位: PHYSICAL OPTICS CORPORATION
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-05-15 至 1998-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2189512
• 关键词: biomedical equipment development; biosensor device; fiber optics; intracellular; microelectrodes; reagent /indicator; single cell analysis; tissue /cell culture

POC proposes to develop a new class of submicroscopic chemical sensors for use in the investigation of cellular physiology. These sensors, which can have characteristic dimensions much smaller than 1 micromole, have the potential for monitoring ionic concentrations, enzyme levels, drug concentrations, and a wide variety of other intracellular chemical properties, with relative ease to the researcher. In the proposed approach, chemically sensitive indicators are immobilized by photopolymerization at the tip of finely tapered optical fibers similar to those used in Near-field Scanning Optical Microscopy (NSOM). The technique could thus combine the ultrafine resolution of NSOM with the sensitivity and specificity of active fiber optic chemical sensors. These sensors will offer significant advantages, including faster response time and minimization of cellular damage, over existing physiological microsensors. During Phase I of the project, POC demonstrated the practicality of this technique by fabricating and testing sensors capable of measuring intracellular pH, calcium ion concentration, sodium ion concentration, and potassium ion concentration. Phase II will see the development of a compact system capable of serving as the basis for a line of commercial submicroscopic sensing products, as well as development of new sensor-tip chemistries both to improve the manufacturability and performance of the tips and to increase the number and types of analytes that can be measured. PROPOSED COMMERCIAL APPLICATION: These sensors will find applications in fields as disparate as fundamental cellular neurophysiology and single- cell drug testing. Beginning with products designed for use in academic and National research laboratories, commercialization of near field optical sensors could ultimately lead to their use in pharmaceutical testing, biotechnology development and process control, and even medical diagnosis and pathology.

POC 提议开发一种新型亚微观化学传感器 用于细胞生理学研究。这些传感器可以 具有远小于 1 微摩尔的特征尺寸，具有 监测离子浓度、酶水平、药物的潜力 浓度和各种其他细胞内化学物质 属性，对于研究人员来说相对容易。在提议的 方法，化学敏感指示剂通过固定 细锥形光纤尖端的光聚合类似于 用于近场扫描光学显微镜（NSOM）的那些。技术 因此可以将 NSOM 的超精细分辨率与灵敏度结合起来 和有源光纤化学传感器的特异性。这些传感器将 提供显着的优势，包括更快的响应时间和 与现有的生理微传感器相比，细胞损伤最小化。 在项目一期中，POC展示了该方案的实用性 通过制造和测试能够测量的传感器的技术 细胞内pH值、钙离子浓度、钠离子浓度，以及 钾离子浓度。第二阶段将开发一个 紧凑的系统能够作为一系列商业产品的基础 亚显微传感产品以及新型传感器尖端的开发 化学物质既提高了可制造性又提高了性能 提示并增加可分析物的数量和类型 测量。 拟议的商业应用：这些传感器将在以下领域找到应用： 基础细胞神经生理学和单细胞神经生理学等不同领域 细胞药物测试。从设计用于学术用途的产品开始 和国家研究实验室，近场商业化 光学传感器最终可能导致其在制药领域的应用 测试、生物技术开发和过程控制，甚至医疗 诊断和病理学。