SUBMICRON CHEMICAL SENSOR FOR INTRACELLULAR APPLICATION

适用于细胞内应用的亚微米化学传感器

• 批准号: 2189509
• 负责人: ROBERT A LIEBERMAN
• 金额: $ 6.27万
• 依托单位: PHYSICAL OPTICS CORPORATION
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-05-15 至 1994-11-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2189509
• 关键词: biomedical equipment development; biosensor device; chemical reaction; fiber optics; intracellular; microelectrodes; reagent /indicator; single cell analysis; tissue /cell culture

POC proposes to develop a new class of submicroscopic chemical sensors for use in the investigation of cellular physiology. These sensors, which can have characteristic dimensions much smaller than l micromole, have the potential for monitoring ionic concentrations, enzyme levels, drug concentrations, and a wide variety of other intracellular chemical properties, with relative ease to the researcher. In the proposed approach, chemically sensitive indicators would be immobilized by photopolymerization at the tip of finely tapered optical fibers similar to those used in Near-field Scanning Optical Microscopy (NSOM). The technique could thus combine the ultrafine resolution of NSOM with the sensitivity and specificity of active fiber optic chemical sensors. The proposed sensors offer significant advantages, including faster response time and minimization of cellular damage, when compared with existing (electrochemical) intracellular sensors. During Phase I of the project, POC will demonstrate the practicality of this technique by fabricating and testing sensors capable of measuring intracellular concentrations of metabolites, ions, or other chemical species of biological interest. Phase II will see expansion into the measurement of other analytes, including biochemical substances such as enzymes and immunochemicals, as well as the development of a portable system capable of serving as the basis for a line of commercial intracellular sensing products.

POC 提议开发一种新型亚微观化学传感器 用于细胞生理学研究。这些传感器可以 具有比 l 微摩尔小得多的特征尺寸，有 监测离子浓度、酶水平、药物的潜力 浓度和各种其他细胞内化学物质 属性，对于研究人员来说相对容易。在提议的 方法，化学敏感指标将被固定 细锥形光纤尖端的光聚合类似于 用于近场扫描光学显微镜（NSOM）的那些。技术 因此可以将 NSOM 的超精细分辨率与灵敏度结合起来 和有源光纤化学传感器的特异性。拟议的 传感器具有显着的优势，包括更快的响应时间和 与现有的相比，细胞损伤最小化 （电化学）细胞内传感器。 在项目第一阶段，POC将展示其实用性 这项技术通过制造和测试能够测量的传感器 代谢物、离子或其他化学物质的细胞内浓度 具有生物学意义的物种。第二阶段将扩大到 其他分析物的测量，包括生化物质，例如 酶和免疫化学物质，以及便携式的开发 系统能够作为一系列商业产品的基础 细胞内传感产品。