INHALED NITRIC OXIDE

这笔赠款建议进行重要的实验室研究，以研究 吸入一氧化氮(NO)的作用和毒理学。我们的实验室有 报道称，吸入低水平的一氧化氮是一种选择性 肺血管扩张剂，可逆转缺氧性肺血管收缩。 NO-HAS合剂用于缺氧新生羊复苏和治疗 弥漫性急性肺损伤。吸入一氧化氮可改善匹配 肺灌流在成人呼吸窘迫中的应用 综合征(ARDS)。吸入NO也是一种快速而深刻的支气管扩张剂 在动物模型中。这笔赠款建议进行实验室研究，以检查 内源性和外源性肺NO产生的相互作用，以及 为了了解是否没有合酶抑制剂可以增加有益的 吸入NO在感染性肺损伤中的作用。低氧新生儿 绵羊呼吸性和代谢性酸中毒对NO诱导的影响 将探索肺血管扩张。对豚鼠的影响 NO将在炎症刺激增强的气道张力上进行检测 (白三烯)。将进行仔细的病理分析 寻找吸入NO的任何毒性影响，最长可达一次 正常成年大鼠和新生大鼠的肺。就这样，我们的 将加强对吸入NO作为一种安全措施的认识 治疗支气管痉挛，肺血管收缩， 弥漫性急性肺部炎症。

This grant proposes important laboratory studies of the mechanisms of action and toxicology of inhaled nitric oxide (NO). Our laboratory has reported that inhaling low levels of nitric oxide is a selective pulmonary vasodilator that reverses hypoxic pulmonary vasoconstriction. NO has mixtures were used to resuscitate hypoxic newborn sheep and treat diffuse acute lung injury. Inhaling nitric oxide improves the matching of pulmonary ventilation with perfusion in the Adult Respiratory Distress Syndrome (ARDS). Inhaled NO is also a rapid and profound bronchodilator in animal models. This grant proposes laboratory studies to examine the interaction of endogenous and exogenous pulmonary NO production, and to learn whether NO synthase inhibitors can augment the beneficial effects of NO inhalation in septic lung injury. In hypoxic newborn sheep the effects of respiratory and metabolic acidosis on NO induced pulmonary vasodilation will be explored. In guinea pigs the effects of NO will be examined on airway tone augmented by inflammatory stimuli (leukotrienes). A careful pathological analysis will be undertaken searching for any toxic effects of inhaling NO for periods up to one month upon normal adult and newborn rat lungs. In this manner our understanding will be enhanced of the safety of NO inhalation as a therapeutic agent in states of bronchospasm, pulmonary vasoconstriction, and diffuse acute pulmonary inflammation.