DUFFY BLOOD GROUP ANTIGEN

达菲血型抗原

• 批准号: 2231144
• 负责人: A OSCAR POGO
• 金额: $ 27.44万
• 依托单位: NEW YORK BLOOD CENTER
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-08-01 至 1998-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2231144
• 关键词: blood group antigens; brain cell; cell cell interaction; chemical binding; complementary DNA; epitope mapping; erythroid stem cell; gene expression; gene targeting; genetic library; glycoprotein structure; glycoproteins; human subject; immunochemistry; immunoelectron microscopy; interleukin 8; laboratory mouse; malaria; membrane transport proteins; molecular site; oligonucleotides; polymerase chain reaction; protein sequence; protein structure function; tissue /cell culture

DESCRIPTION: Having cloned the gene for the Duffy protein, the Investigators propose to: 1. determine the topology of the amino and carboxyl ends by immunochemical binding or double labeling immunoelectromicroscopy (an approach that is not intuitively obvious; 2. determine the antibody and malarial binding sites on the external portion of the molecule; 3. determine the sequence of non- erythroid Duffy protein; 4. determine by immunochemistry which cells produce Duffy proteins; 5. determine the chemikine binding requirements; 6. find and clone the mouse equivalent gene; and 7. study the interactions of Duffy and Rh since a Duffy antigen (Fy5) depends upon the presence of Rh proteins for its expression. Long-term goals consist of the following: 1. determination of the function of the glycoprotein; 2. determine the significance of a Duffy like protein in the brain; 3. determine the sequence the binds to the merozoites of malaria organisms; 4. determine whether the Duffy antigen is a transporter; and 5. design mouse knock-out models to determine the significance of the Duffy protein.

在克隆了达菲蛋白的基因后