DUFFY BLOOD GROUP ANTIGEN

达菲血型抗原

• 批准号: 2460088
• 负责人: A OSCAR POGO
• 金额: $ 29.68万
• 依托单位: NEW YORK BLOOD CENTER
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-08-01 至 1998-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2460088
• 关键词: blood group antigens; brain cell; cell cell interaction; chemical binding; complementary DNA; epitope mapping; erythroid stem cell; gene expression; gene targeting; genetic library; glycoprotein structure; glycoproteins; human subject; immunochemistry; immunoelectron microscopy; interleukin 8; laboratory mouse; malaria; membrane transport proteins; molecular site; oligonucleotides; polymerase chain reaction; protein sequence; protein structure function; tissue /cell culture

DESCRIPTION: Having cloned the gene for the Duffy protein, the Investigators propose to: 1. determine the topology of the amino and carboxyl ends by immunochemical binding or double labeling immunoelectromicroscopy (an approach that is not intuitively obvious; 2. determine the antibody and malarial binding sites on the external portion of the molecule; 3. determine the sequence of non- erythroid Duffy protein; 4. determine by immunochemistry which cells produce Duffy proteins; 5. determine the chemikine binding requirements; 6. find and clone the mouse equivalent gene; and 7. study the interactions of Duffy and Rh since a Duffy antigen (Fy5) depends upon the presence of Rh proteins for its expression. Long-term goals consist of the following: 1. determination of the function of the glycoprotein; 2. determine the significance of a Duffy like protein in the brain; 3. determine the sequence the binds to the merozoites of malaria organisms; 4. determine whether the Duffy antigen is a transporter; and 5. design mouse knock-out models to determine the significance of the Duffy protein.

描述：克隆了达菲蛋白的基因后， 研究人员建议：1。确定氨基的拓扑结构 通过免疫化学结合或双标记， 免疫电子显微镜（一种直观上不明显的方法; 2.确定抗体和疟疾的结合位点， 分子的外部部分; 3.确定非- 红系Duffy蛋白; 4.通过免疫化学确定哪些细胞 产生达菲蛋白; 5.确定化学因子结合要求; 6.找到并克隆小鼠等价基因; 7.研究 Duffy和Rh的相互作用，因为Duffy抗原（Fy 5）依赖于 在Rh蛋白的存在下进行表达。 长期 目标包括以下内容：1。函数的确定 糖蛋白; 2.确定达菲的重要性 就像大脑中的蛋白质一样; 3.确定结合到 疟疾生物体的裂殖子; 4.确定达菲是否 抗原是转运蛋白;和5.设计小鼠敲除模型， 确定达菲蛋白的重要性

项目成果

Detection of duffy antigen in the plasma membranes and caveolae of vascular endothelial and epithelial cells of nonerythroid organs

• DOI: 10.1182/blood.v89.2.701
• 发表时间: 1997-01-15
• 期刊: BLOOD
• 影响因子: 20.3
• 作者: Chaudhuri, A;Nielsen, S;Pogo, AO
• 通讯作者: Pogo, AO