RETICULOSPINAL & VESTIBULOSPINAL CONTROL OF BACK MUSCLES

下行系统包括延髓网状脊髓和外侧网状脊髓 前庭脊髓神经元已被证明负责促进深 背部肌肉收缩在充分分析的生殖行为， 脊柱前凸 这些下行系统的性质和深背 肌肉与脊柱前凸行为要求显著一致， 已经被证明可以进行生理和细胞分析。 在刺激下，这些神经元中发生的化学反应， 电激活导致背部深层肌肉 宫缩？ 我们将使用以下内容回答此问题的一部分 应用免疫细胞化学和原位杂交技术检测早期乳腺癌组织中 反应基因，因为这些基因对神经元的 刺激. 我们将在延髓网状脊髓和外侧 前庭脊髓细胞，以及深背部肌肉运动神经元， 将表达与背部肌肉收缩相关， 脑干刺激参数 最重要的是，fos表达将 在依赖使用的促进过程中，以及 与躯体感觉输入或中脑中央灰质的相互作用 刺激. 将用c-jun和NGFI-A进行平行研究。 我们能发现神经元中c-fos表达的任何后果吗？我们可以 在特定的神经元组中实现过表达， 病毒载体，并且可以使用反义序列实现低表达。 这些技术都是在我们的实验室里完成的。我们将研究 这些中的每一个对于受刺激的深背部肌肉EMG的结果为 以及随后的延髓脑啡肽和P物质表达 网状细胞 这些实验将提供新的信息，说明早期反应基因 可以参与神经元刺激，导致容易研究 生殖行为

The descending systems comprised of medullary reticulospinal and lateral vestibulospinal neurons have been shown responsible for facilitating deep back muscle contraction in the well analyzed reproductive behavior, lordosis. Properties of these descending systems and the deep back muscles are remarkably congruent with lordosis behavior requirements, and have proven unusually accessible to physiological and cellular analysis. What is occurring chemically in these neurons upon stimulation, under conditions where electrical activation leads to deep back muscle contraction? We will answer part of this question using immunocytochemistry and in situ hybridization for expression of early response genes since these are exquisitely responsive to neuronal stimulation. We will study c-fos in medullary reticulospinal and lateral vestibulospinal cells, as well as deep back muscle motoneurons, to correlate expression with back muscle contraction as a function of brainstem stimulation parameters. Most important, fos expression will be followed during use-dependent facilitation, as well as during interactions with somatosensory input or midbrain central gray stimulation. Parallel studies will be carried out with c-jun and NGFI-A. Can we discover any consequences of c-fos expression in neurons? We can achieve overexpression in specific neuronal groups using our defective viral vector and can achieve underexpression using antisense sequences. Each of these techniques has been worked out in our lab. We will study the consequences of each of these for stimulated deep back muscle EMG as well as for subsequent enkephalin and substance P expression in medullary reticular cells. These experiments will give new information on how early response genes could participate in neuronal stimulation leading to an easily studied reproductive behavior.