RETICULOSPINAL & VESTIBULOSPINAL CONTROL OF BACK MUSCLES

网状脊髓

• 批准号: 2244603
• 负责人: Donald W. Pfaff
• 金额: $ 9.4万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-02-01 至 1995-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2244603
• 关键词: antisense nucleic acid; brain stem; electromyography; enkephalins; gene expression; immunocytochemistry; in situ hybridization; laboratory rat; muscle contraction; neural facilitation; neurochemistry; neurons; nucleoproteins; reticulospinal tract; sex behavior; substance P; transfection /expression vector; vestibular pathway

The descending systems comprised of medullary reticulospinal and lateral vestibulospinal neurons have been shown responsible for facilitating deep back muscle contraction in the well analyzed reproductive behavior, lordosis. Properties of these descending systems and the deep back muscles are remarkably congruent with lordosis behavior requirements, and have proven unusually accessible to physiological and cellular analysis. What is occurring chemically in these neurons upon stimulation, under conditions where electrical activation leads to deep back muscle contraction? We will answer part of this question using immunocytochemistry and in situ hybridization for expression of early response genes since these are exquisitely responsive to neuronal stimulation. We will study c-fos in medullary reticulospinal and lateral vestibulospinal cells, as well as deep back muscle motoneurons, to correlate expression with back muscle contraction as a function of brainstem stimulation parameters. Most important, fos expression will be followed during use-dependent facilitation, as well as during interactions with somatosensory input or midbrain central gray stimulation. Parallel studies will be carried out with c-jun and NGFI-A. Can we discover any consequences of c-fos expression in neurons? We can achieve overexpression in specific neuronal groups using our defective viral vector and can achieve underexpression using antisense sequences. Each of these techniques has been worked out in our lab. We will study the consequences of each of these for stimulated deep back muscle EMG as well as for subsequent enkephalin and substance P expression in medullary reticular cells. These experiments will give new information on how early response genes could participate in neuronal stimulation leading to an easily studied reproductive behavior.

由延髓、网状、脊髓和外侧组成的下行系统 前庭脊髓神经元已被证明负责促进深部 背部肌肉收缩在分析清楚的生殖行为中， 前凸。这些下行系统和背部深处的性质 肌肉与前凸行为要求非常一致，而且 已经被证明是非常容易被生理和细胞分析获得的。 在刺激下，这些神经元在化学上发生了什么？ 电激活导致背部深部肌肉的条件 宫缩？我们将使用以下内容回答此问题的一部分 免疫细胞化学和原位杂交检测早期肿瘤细胞的表达 反应基因，因为这些基因对神经元的反应非常灵敏 刺激。我们将研究c-fos在延髓网状脊髓和外侧的分布。 前庭脊髓细胞以及背部深部肌肉运动神经元 表达与背部肌肉收缩作为函数的相关性 脑干刺激参数。最重要的是，FOS表达将 在依赖使用的促进过程中以及在 与躯体感觉输入或中脑中央灰质的相互作用 刺激。将与c-Jun和NGFI-A进行平行研究。 我们能发现c-fos在神经元中的表达有什么后果吗？我们可以的 利用我们的缺陷基因在特定神经元群中实现过度表达 病毒载体，并可利用反义序列实现低表达。 这些技术中的每一种都是我们实验室研究出来的。我们会研究 其中每一项对刺激背部深部肌肉肌电的影响 以及随后脑啡肽和P物质在延髓中的表达 网状细胞。 这些实验将提供关于早期反应基因如何 可以参与神经刺激，导致一种容易学习的 生殖行为。