REACTIVATION OF TUBERCULOSIS BY RESTRAINT STRESS

束缚压力导致结核病复发

• 批准号: 2255401
• 负责人: BRUCE S ZWILLING
• 金额: $ 21.31万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2255401
• 关键词: Mycobacterium tuberculosis; T lymphocyte; bactericidal immunity; cell mediated cytotoxicity; cellular immunity; corticosterone; cytokine; flow cytometry; gene expression; hypothalamic pituitary axis; immunosuppression; laboratory mouse; leukocyte activation /transformation; lymphocyte proliferation; macrophage; microorganism growth; neuroimmunomodulation; nitric oxide; pituitary adrenal axis; polymerase chain reaction; psychological stressor; restraint; stress; sympathetic nervous system; tuberculosis

The incidence of tuberculosis in the United States has increased during the past 10 years and tuberculOsis continues to be a significant public health problem world wide. Most individuals who are infected with Mycobacterium tuberculosis develop a cell mediated immune response and control the disease. Infected individuals stand a 10% lifetime chance of the disease reactivating. Conventional wisdom has attributed reactivation to a temporary suppression Of the immune response which allows the microorganisms to grow. Among the factors thought to be responsible for reactivation of Mycobacterial growth are infection with the human immunodeficiency virus, immunosenesence due to aging, protein malnutrition associated with chronic alcoholism and stress. The purpose of this investigation is to evaluate the effects of stress on the reactivation of Mycobacterium tuberculosis growth in a mouse model. We will test the hypothesis that stress serves as a cofactor in reactivation by altering T cell and macrophage mediated control of Mycobacterial growth. Our specific aims are: l. To determine the effect of restraint stress on reactivation of the growth of Mycobacterium tuberculosis; 2. To determine the changes in T cell mediated immunity as a result of restraint stress that results in the growth of Mycobacterium tuberculosis, and 3. To determine the changes in macrophage mediated control of Mycobacterium tuberculosis growth that results from restraint stress.

美国结核病的发病率在2000年有所上升。 过去10年，结核病仍然是一个重要的公共卫生问题， 全世界的问题。 大多数感染分枝杆菌的人 结核病发展细胞介导的免疫反应，并控制 疾病 受感染的人一生中有10%的机会患病 重新激活 传统观点将重新激活归因于 暂时抑制免疫反应， 微生物生长。 在被认为是导致 重新激活分枝杆菌的生长是人类感染 免疫缺陷病毒，衰老性免疫衰老，蛋白质营养不良 与慢性酒精中毒和压力有关 这样做的目的 研究的目的是评估压力对重新激活的影响， 结核分枝杆菌在小鼠模型中的生长。 我们将测试 假设压力作为辅助因子通过改变T 细胞和巨噬细胞介导的分枝杆菌生长控制。 我们的具体 目标是：l.确定束缚应力对再活化的影响 结核分枝杆菌的生长; 2.为了确定这些变化 在T细胞介导的免疫中，由于束缚应激，结果 结核分枝杆菌的生长， 3.确定 巨噬细胞介导的结核分枝杆菌生长控制的变化 这是束缚压力造成的