ETHANOL--SUPPRESSION OF MYCOBACTERIAL RESISTANCE

大量饮酒与呼吸系统疾病风险增加有关。 疾病和结核病，并可能与增加 与人类免疫缺陷相关分枝杆菌病发病率 病毒感染。 在死于艾滋病的人中， 感染了鸟分枝杆菌 这项工作是一个延伸 在我的实验室里的工作表明， MHCII类糖蛋白的表达 单核细胞可能与先天激活状态的差异有关 巨噬细胞的数量和对分枝杆菌感染的抗性差异。 为了实现我们的目标，我们将利用一个模型， 利用小鼠的同类品系的分枝杆菌病， 对分枝杆菌的体内生长具有抗性或敏感性。 研究 已经表明，消耗乙醇或治疗巨噬细胞， 乙醇体外抑制抗分枝杆菌 阻力 为了了解乙醇对增加的 对分枝杆菌生长的敏感性，我们将：确定 乙醇消耗量对脾脏中鸟分枝杆菌生长的影响 和肺的感染小鼠;确定乙醇消耗量的影响， 巨噬细胞介导的抗分枝杆菌机制，并确定 体外乙醇处理对抗分枝杆菌活性的影响 脾和肺巨噬细胞，包括它们对细胞因子的反应。

Heavy use of alcohol is associated with an increase risk of respiratory disease and of tuberculosis and may be associated with the increased incidence of Mycobacterial disease associated with human immunodeficiency virus infection. As many as 50% of the individuals who have died of AIDS have been infected with Mycobacterium avium. This work is an extension of work in my laboratory that has shown that the differential expression of MHC class II glycoproteins by both murine macrophages and human monocytes may be related to differences in the innate state of activation of macrophages and differences in resistance to Mycobacteria] infection. To accomplish our goals we will take advantage of a model for Mycobacterial disease that utilizes congenic strains of mice that are resistant or susceptible to the in vivo growth of Mycobacteria. Studies have shown that consumption of ethanol or treatment of macrophages in vitro with ethanol results in the suppression of anti-mycobacterial resistance. To understand the role of ethanol on the increased susceptibility to Mycobacterial growth we will: Determine the effect of ethanol consumption on the growth of Mycobacterium avium in the spleen and lung of infected mice; determine the effect of ethanol consumption on macrophage mediated anti-mycobacterial mechanisms and determine the effect of in vitro ethanol treatment on the antimycobacterial activity of splenic and lung macrophages including their response to cytokines.