IN VITRO BIOCHEMICAL DIFFERENTIATION OF NERVE CELLS

调查的目的将是阐明生物化学的本质 细胞间的相互作用对胚胎发育和分化的影响 中枢神经系统。新生小鼠的重组和表面培养 小脑将用于监测生化和形态发育 。 胞外蛋白水解酶，主要是纤溶酶原激活物在 颗粒细胞在体外和体内的迁移将使用 小鼠组织型纤溶酶原特异性蛋白水解物和抗体 激活剂。将监测组织纤溶酶原激活物基因转录 在小脑发育过程中和一个新提出的神经细胞表面 组织型纤溶酶原激活剂受体的特性。行动 胞外蛋白水解酶是一种重要的机制 E 胚胎发育和神经发育过程中细胞和轴突生长锥体的迁移 组织发生及其调控可能对神经产生重要影响 再生。

Investigations will be directed at elucidating the biochemical nature of cell-cell interactions that influence development and differentiation in th central nervous system. Reaggregate and surface cultures of neonatal mouse cerebellum will be used to monitor biochemical and morphological developmen . The role of extracellular proteases, primarily plasminogen activator, in granule cell migration will be examined both in vitro and in vivo using specific proteolytic inhibitors and antibodies to murine tissue plasminogen activator. Tissue plasminogen activator gene transcription will be monitore during cerebellar development and a newly proposed neuronal cell surface receptor for tissue plasminogen activator will be characterized. The action of extracellular proteases appears to be an important mechanism to facilita e cell and axonal growth cone migration during embryogenesis and neural histogenesis, and its regulation may have important consequences for neural regeneration.