IN VITRO BIOCHEMICAL DIFFERENTIATION OF NERVE CELLS

研究将针对阐明的生化性质， 细胞间相互作用影响发育和分化， 中枢神经系统新生小鼠的再聚集体和表面培养 小脑将用于监测生化和形态发育， . 细胞外蛋白酶，主要是纤溶酶原激活物，在 颗粒细胞迁移将在体外和体内使用 特异性蛋白水解抑制剂和鼠组织纤溶酶原抗体 活化剂。将监测组织纤溶酶原激活物基因转录 在小脑发育过程中，新提出的神经元细胞表面 将表征组织纤溶酶原激活物的受体。的行动 细胞外蛋白酶似乎是一个重要的机制，以促进 e 在胚胎发生和神经发育过程中细胞和轴突生长锥的迁移 组织发生及其调节可能对神经系统的发育有重要影响。 再生

Investigations will be directed at elucidating the biochemical nature of cell-cell interactions that influence development and differentiation in th central nervous system. Reaggregate and surface cultures of neonatal mouse cerebellum will be used to monitor biochemical and morphological developmen . The role of extracellular proteases, primarily plasminogen activator, in granule cell migration will be examined both in vitro and in vivo using specific proteolytic inhibitors and antibodies to murine tissue plasminogen activator. Tissue plasminogen activator gene transcription will be monitore during cerebellar development and a newly proposed neuronal cell surface receptor for tissue plasminogen activator will be characterized. The action of extracellular proteases appears to be an important mechanism to facilita e cell and axonal growth cone migration during embryogenesis and neural histogenesis, and its regulation may have important consequences for neural regeneration.