ELECTRICAL MEASUREMENT OF CELL MOTILITY IN VITRO

体外细胞活力的电测量

• 批准号: 2096634
• 负责人: CHARLES R KEESE
• 金额: $ 34.26万
• 依托单位: APPLIED BIOPHYSICS, INC.
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-07-10 至 1998-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2096634
• 关键词: adenocarcinoma; biomedical equipment development; biopsy; biosensor device; cell adhesion; cell motility; electrical impedance; electrical measurement; electrodes; human subject; male; metastasis; method development; oncogenes; prognosis; prostate neoplasms; tissue /cell culture; transfection; video microscopy

Prostatic carcinoma constitutes one of the most prevalent forms of cancer in men. Previous research has shown the propensity of these tumor cells to metastasize is correlated with their motility in tissue culture, yet it has not been possible to devise practical prognostic assays based on this fact. The goal of this research is to employ an innovative cell motility measurement referred to as ECIS (electric cell-substrate impedance sensing) as the basis of an assay to determine the metastatic potential of prostate tumors. Phase I research with Dunning sublines of murine prostatic adenocarcinoma, demonstrated several significant correlations of ECIS data and metastatic potential of the sublines Phase II will extend these measurements to characterize human prostatic cancer cell lines. From this research, the most effective ECIS protocols to discriminate metastatic abilities will be selected and applied to analyze primary cultures established from prostate tumor outgrowths. A device will be designed, fabricated, and tested to simplify clinical assays by combining both formation of outgrowths from prostate needle biopsies and ECIS assays. The commercial opportunities of a clinical method for prognosis of prostate cancer are clear. In addition, publication of this work will promote the ECIS method as a general purpose research tool to study cell motility.

前列腺癌是最常见的癌症之一。 在男人身上。先前的研究表明，这些肿瘤细胞倾向于 转移与它们在组织培养中的运动性有关，但它 不可能在此基础上设计出实用的预后评估方法 事实。这项研究的目标是利用一种创新的细胞运动性 测量称为ECIS(电池-衬底阻抗 感测)作为确定肿瘤转移潜能的检测的基础 前列腺癌。第一阶段小鼠Dunning亚系研究 前列腺癌，表现出几个显著的相关性 ECIS数据和亚系第二期转移潜能将延长 这些测量用来表征人类前列腺癌细胞系。从… 本研究，对ECIS协议进行最有效的区分 将选择转移能力并将其应用于分析初级 从前列腺癌生长组织建立的培养物。一个装置将会是 设计、制造和测试以简化临床分析 前列腺针组织活检和ECIS形成的突出物 化验。一种临床预后方法的商机 前列腺癌是明确的。此外，这项工作的出版将 推广ECIS方法作为研究细胞的通用研究工具 能动性。