ELECTRODE ARRAYS IN TISSUE CULTURE

这项拟议的研究将把一个概念从实验室设备转化为 适合商业应用的仪器。当哺乳动物细胞 在小金膜电极上培养，这些电极的阻抗 用微弱的交流信号测量反映了细胞的行为。一 可以实时检测细胞形态的变化，包括扩散 和细胞运动。这些电极的高效生产是 开发其商业版本的主要技术障碍 乐器。第一阶段演示了光刻是一种 很好的方法。第二阶段将把这项研究扩展到制造 可供使用者使用且适合组织培养的电极阵列 申请。此外，电子仪器将被 在一个窗口中简化和进一步开发和重写软件 格式化。 随着第二阶段的完成，首次将有一台仪器 存在可以电子探测细胞的细微形态变化的装置 在文化上。可获得药物和毒素的剂量-反应曲线 由于几乎没有体力劳动和新的医疗应用，可能会推动 仪器投入临床使用。

The proposed research will take a concept from a laboratory device to an instrument suitable for commercial application. When mammalian cells are cultured on small gold film electrodes, the impedance of these electrodes measured with a weak AC signal reflects the behavior of the cells. One can detect, in real time, changes in cell morphology including spreading and cell motion. The efficient production of these electrodes is the main technical hurdle in developing a commercial version of this instrument. Phase I demonstrated that photoresist lithography is an excellent approach. Phase II will extend this research to fabricate electrode arrays that are user ready and suitable for tissue culture applications. In addition, the electrical instrumentation will be simplified and the software further developed and rewritten in a window format. With the completion of Phase II, for the first time, an instrument will exist that can electrically probe subtle morphological changes of cells in culture. Dose-response curves to drugs and toxins can be obtained with little manual labor and novel medical applications might move the instrument into clinical use.