ELECTRICAL APPROACH FOR IN VITRO TOXICOLOGY

体外毒理学的电气方法

• 批准号: 3496752
• 负责人: CHARLES R KEESE
• 金额: $ 5万
• 依托单位: APPLIED BIOPHYSICS, INC.
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-30 至 1993-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3496752
• 关键词: alternatives to animals in research; biosensor device; cell adhesion; cell cell interaction; cell cycle; cell migration; cell motility; clone cells; cytotoxicity; electrical impedance; electrical measurement; electrodes; method development; morphology; statistics /biometry; tissue /cell culture; toxicant screening

We have been developing a new means to electrically study animal cells grown in tissue culture and intend to make a commercial device suitable for use in the medical/biological research and testing market. The instrumental method, referred to as ECIS (electric cell-substrate impedance sensor), is a non-invasive technique that measures several parameters related to cell behavior, some of which have previously been difficult to quantify. These include adhesion and spreading, cell growth, cell motility, and the barrier function of cell monolayers. The objective of the proposed research is to test the feasibility of using this approach to evaluate the cytotoxicity of compounds. The cell behaviors detected by ECIS are complex and, hence, require the integrated activities of many cellular systems. It is hoped their measurement will be particularly useful in screening potentially toxic compounds without the use of whole animals. Specifically, extensive ECIS measurements will be carried out using different cell lines exposed to varying concentrations of compounds with well documented cytotoxicity. These dose-response data will be statistically compared with existing data both from established cell culture assays and from whole animals. This aspect of the research will be carried out in collaboration with the Center for Alternatives to Animal Testing at Johns Hopkins University.

我们一直在开发一种新的方法来电学研究动物细胞 在组织培养中生长，并打算制造适合于 用于医学/生物学研究和测试市场。 的 仪器方法，称为ECIS（细胞-基质阻抗 传感器）是一种非侵入性技术， 与细胞行为有关，其中一些以前很难 量化。 这些包括粘附和铺展、细胞生长、细胞增殖、细胞凋亡、细胞增殖、细胞凋亡、细胞 运动性和细胞单层的屏障功能。 本研究的目的是测试使用 该方法用于评价化合物的细胞毒性。 细胞 ECIS检测到的行为是复杂的，因此需要集成 许多细胞系统的活动。 希望他们的测量结果能 特别适用于筛选潜在的有毒化合物， 使用整个动物。 具体而言，将使用以下方法进行广泛的ECIS测量： 暴露于不同浓度的化合物的不同细胞系， 细胞毒性证据充分。 这些剂量反应数据将是 统计学上与现有的数据进行比较， 培养测定和来自整个动物。 这方面的研究将是 与动物替代品中心合作开展 约翰霍普金斯大学的测试。