EMBRYONIC AND EARLY POSTNATAL WEAVER MICE

胚胎和产后早期织鼠

• 批准号: 2262005
• 负责人: JAMES H. MILLONIG
• 金额: $ 2.37万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-04-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2262005
• 关键词: EMBRYONIC; EARLY; POSTNATAL; WEAVER; MICE

This research proposal combines molecular genetics with neurobiology and cell biology to define the role of the weaver gene in cerebellar development. Mouse microsatellite markers that map close to the gene will be used to identify embryonic weaver homozygotes. This technology will be used to examine the development of the cerebellum in weaver embryos prior to the apparent morphological manifestations of the mutant. In situ and immunocytochemistry analysis will be used to answer these questions. In addition, reaggregate cultures with embryonic weaver granule cells will determine the earliest time in granule cell development that the mutation has an effect. Rescue of these cultures with wild type granule cells will determine the time in cerebellar development that the weaver ligand is present.

这项研究计划将分子遗传学与神经生物学相结合， 细胞生物学来确定韦弗基因在小脑中的作用 发展 小鼠的微卫星标记， 将被用来鉴定胚胎织工纯合子。 这项技术 将被用来研究韦弗小脑的发育 在突变体的明显形态学表现之前的胚胎。 原位和免疫细胞化学分析将用于回答这些问题 问题. 另外，用胚胎织纹虫 颗粒细胞将决定最早进入颗粒细胞的时间 突变会产生影响。 拯救这些文化 与野生型颗粒细胞将确定小脑中的时间 发现了韦弗配体。