NEUROGENESIS IN THE MAMMALIAN CEREBELLUM

该项目的总体目标是提供关于 控制颗粒细胞命运特化的机制 小脑皮质 在拟议的研究中，我们将研究，在 细胞和分子水平，控制分化的信号 来源于外胚层（EGL）的前体细胞 小鼠小脑皮质，使用我们开发的体外模型系统， 实验室 将讨论两个问题。 首先，我们将确定 生长因子，特别是那些高水平表达的， 在小脑发育中（bFGF、NT 3和BDNF）调节颗粒细胞 规范. 第二，提供颗粒细胞的遗传分析， 确定，我们将分析EGL前体细胞从神经 一种具有颗粒细胞表型缺陷突变型小鼠--Weaver 分化，包括神经突生长和胶质引导的失败 迁移

The overall goal of this project is to provide information on the mechanisms that control the specification of granule cell fate in the cerebellar cortex. In the proposed research, we will examine, at the cellular and molecular levels, the signals that control the differentiation of precursor cells derived from the external germinal layer (EGL) of the mouse cerebellar cortex, using an in vitro model system developed in our laboratory. Two issues will be addressed. First, we will determine whether growth factors, in particular those with high levels of expression in developing cerebellum (bFGF, NT3 and BDNF) regulate granule cell specification. Second, to provide a genetic analysis of granule cell determination, we will analyze EGL precursor cells from the neurological mutant mouse weaver, an animal with phenotypic defects in granule cell differentiation, including a failure of neurite outgrowth and glial-guided migration.