SEARCH FOR ANTINEURAL ANTIBODIES IN MULTIPLE SCLEROSIS

多发性硬化症(MS)是一种炎症性脱髓鞘疾病。 人类中枢神经系统的病因和发病机制不明。 尽管多发性硬化症的发病机制被怀疑是自身免疫基础，但 引发多发性硬化脱髓鞘的疑似抗原(S)是 尽管进行了多年的密集研究，但仍不得而知。最近，它一直在 证明糖脂和糖蛋白是重要的抗原 很大比例的脱髓鞘神经病患者 与单克隆性IgM病和格林-巴利病有关 综合症。我们假设抗复杂糖脂的自身抗体和 蛋白质也可能存在于MS中，并可能在 MS的发病机制我们建议系统地检测血清和 大量多发性硬化症患者和对照组的脑脊液， 存在针对复合糖脂和蛋白质的自身抗体 抗原。为此，我们将使用三种敏感的方法：免疫印迹、 酶联免疫吸附试验(ELISA法)和薄层色谱- 免疫染色技术。我们将尝试确定两国的关系 系列血清检测临床病程自身抗体滴度的研究 临床稳定的复发缓解型多发性硬化症患者的标本 复发进展型和慢性进展型多发性硬化患者。识别 抗原可以提供宝贵的信息，以建立 并可能导致更好的治疗方法的发展。

Multiple sclerosis (MS) is an inflammatory demyelinating disease of the human central nervous system of unknown etiology and pathogenesis. Although an autoimmune basis for the pathogenesis of MS is suspected, the putative antigen(s) responsible for initiating demyelination in MS is unknown despite many years of intensive research. Recently it has been demonstrated that glycolipids and glycoproteins are important antigens in a large proportion of patients with the demyelinating neuropathy associated with monoclonal IgM gammopathy and with the Guillain-Barre' syndrome. We hypothesize that autoantibodies to complex glycolipids and proteins may also be present in MS, and may play a role in the pathogenesis of MS. We propose to systematically test sera and cerebrospinal fluid from a large number of patients with MS and controls, for the presence of autoantibodies to complex glycolipid and protein antigens. To do this, we will use three sensitive methods: immunoblots, enzyme-linked immunosorbent assay (ELISA), and a thin-layer chromatogram- immunostaining technique. We will attempt to determine the relationship of autoantibody titer to clinical disease course by examining serial serum specimens from MS patients with relapsing-remitting, clinically stable, relapsing progressive and chronic progressive MS patients. Identifying the antigen may provide invaluable information for establishing the pathogenesis, and could lead to the development of better therapies.