SEARCH FOR ANTINEURAL ANTIBODIES IN MULTIPLE SCLEROSIS

多发性硬化症（MS）是一种炎症性脱髓鞘疾病， 人类中枢神经系统病因和发病机制不明。 虽然MS的发病机制的自身免疫基础被怀疑， 负责启动MS脱髓鞘的假定抗原是 尽管经过多年的深入研究，最近已经 表明糖脂和糖蛋白是重要的抗原， 大部分脱髓鞘性神经病患者 与单克隆IgM丙种球蛋白病和格林-巴利综合征相关 综合征我们假设，自身抗体的复杂糖脂和 蛋白质也可能存在于MS中，并且可能在MS中起作用。 MS的发病机制。我们建议系统地测试血清， 来自大量MS患者和对照的脑脊液， 对于复合糖脂和蛋白质的自身抗体的存在 抗原为此，我们将使用三种灵敏的方法：免疫印迹， 酶联免疫吸附试验（ELISA）和薄层色谱图- 免疫染色技术。我们将尝试确定 系列血清自身抗体效价与临床病程的关系 复发缓解型、临床稳定型 复发进行性和慢性进行性MS患者。识别 抗原可以提供宝贵的信息， 发病机制，并可能导致更好的治疗方法的发展。

Multiple sclerosis (MS) is an inflammatory demyelinating disease of the human central nervous system of unknown etiology and pathogenesis. Although an autoimmune basis for the pathogenesis of MS is suspected, the putative antigen(s) responsible for initiating demyelination in MS is unknown despite many years of intensive research. Recently it has been demonstrated that glycolipids and glycoproteins are important antigens in a large proportion of patients with the demyelinating neuropathy associated with monoclonal IgM gammopathy and with the Guillain-Barre' syndrome. We hypothesize that autoantibodies to complex glycolipids and proteins may also be present in MS, and may play a role in the pathogenesis of MS. We propose to systematically test sera and cerebrospinal fluid from a large number of patients with MS and controls, for the presence of autoantibodies to complex glycolipid and protein antigens. To do this, we will use three sensitive methods: immunoblots, enzyme-linked immunosorbent assay (ELISA), and a thin-layer chromatogram- immunostaining technique. We will attempt to determine the relationship of autoantibody titer to clinical disease course by examining serial serum specimens from MS patients with relapsing-remitting, clinically stable, relapsing progressive and chronic progressive MS patients. Identifying the antigen may provide invaluable information for establishing the pathogenesis, and could lead to the development of better therapies.