MECHANISM OF ENZYME INHIBITION BY PHARMACOLOGICAL AGENTS
MECHANISM OF ENZYME INHIBITION BY PHARMACOLOGICAL AGENTS
批准号:
2269863
负责人:
JOHN J. COLICELLI
金额:
$6.3万
依托单位国家:
美国
项目类别:
财政年份:
1993
资助国家:
美国
项目状态:
已结题
起止时间:
1993-08-01 至 1996-07-31
中文摘要
此建议的目标是定义一个
英文摘要
The objective of this proposal is to define the interaction between an
enzyme and a highly specific competitive inhibitor. The enzyme being
studied is a mammalian cAMP-specific isozyme of phosphodiesterase (PDE)
that is strongly implicated in learning and memory functions. Inhibitors
of this enzyme are pharmacologically useful in the treatment of some
human pathologies.
The work involves the use of a novel in vivo drug-resistance selection
for mutants of a mammalian enzyme expressed in yeast. This reconstitution
of a mammalian gene product into yeast cells results in a simplified
system and a mechanism for the isolation of rare drug resistant mutants.
The selection to be used is designed to isolate mammalian PDE mutants
that are incapable of interacting with a competitive inhibitor
(rolipram), but are not affected in substrate (cAMP) binding or
hydrolysis.
This work should lead to a better understanding of the nature of the
interaction between a mammalian PDE enzyme and a pharmacologically useful
drug. In addition, the methods employed in this research should prove
useful in both the design and testing of more potent inhibitors of this
enzyme. The methodology will also be extended to the study of other
mammalian enzymes. These new techniques could provide an invaluable tool
for the analysis of a wide variety of inhibitor-enzyme interactions.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
A Novel Screen for Compounds that Outflank BCR-ABL Drug-Resistance
-
批准号:8067981
-
项目类别:
-
资助金额:$30.59万
-
财政年份:2009
-
负责人:JOHN J. COLICELLI
-
依托单位:
A Novel Screen for Compounds that Outflank BCR-ABL Drug-Resistance
-
批准号:7725809
-
项目类别:
-
资助金额:$31.54万
-
财政年份:2009
-
负责人:JOHN J. COLICELLI
-
依托单位:
Mechanism of RIN1 Signaling in Neuronal Plasticity
-
批准号:6773149
-
项目类别:
-
资助金额:$28.57万
-
财政年份:2003
-
负责人:JOHN J. COLICELLI
-
依托单位:
Mechanism of RIN1 Signaling in Neuronal Plasticity
-
批准号:7259412
-
项目类别:
-
资助金额:$27.09万
-
财政年份:2003
-
负责人:JOHN J. COLICELLI
-
依托单位:
Mechanism of RIN1 Signaling in Neuronal Plasticity
-
批准号:6681142
-
项目类别:
-
资助金额:$28.57万
-
财政年份:2003
-
负责人:JOHN J. COLICELLI
-
依托单位:
Mechanism of RIN1 Signaling in Neuronal Plasticity
-
批准号:7067644
-
项目类别:
-
资助金额:$27.9万
-
财政年份:2003
-
负责人:JOHN J. COLICELLI
-
依托单位:
Mechanism of RIN1 Signaling in Neuronal Plasticity
-
批准号:6910730
-
项目类别:
-
资助金额:$28.57万
-
财政年份:2003
-
负责人:JOHN J. COLICELLI
-
依托单位:
MECHANISM OF ENZYME INHIBITION BY PHARMACOLOGICAL AGENTS
-
批准号:2714522
-
项目类别:
-
资助金额:$10.44万
-
财政年份:1993
-
负责人:JOHN J. COLICELLI
-
依托单位:
MECHANISM OF ENZYME INHIBITION BY PHARMACOLOGICAL AGENTS
-
批准号:2037678
-
项目类别:
-
资助金额:$10.14万
-
财政年份:1993
-
负责人:JOHN J. COLICELLI
-
依托单位:
MECHANISM OF ENZYME INHIBITION BY PHARMACOLOGICAL AGENTS
-
批准号:3418801
-
项目类别:
-
资助金额:$6.06万
-
财政年份:1993
-
负责人:JOHN J. COLICELLI
-
依托单位:
MECHANISM OF ENZYME INHIBITION BY PHARMACOLOGICAL AGENTS
-
批准号:2269864
-
项目类别:
-
资助金额:$6.56万
-
财政年份:1993
-
负责人:JOHN J. COLICELLI
-
依托单位:
MECHANISM OF ENZYME INHIBITION BY PHARMACOLOGICAL AGENTS
-
批准号:2891880
-
项目类别:
-
资助金额:$10.67万
-
财政年份:1993
-
负责人:JOHN J. COLICELLI
-
依托单位:
EFFECTORS AND REGULATORS OF NORMAL AND ONCOGENIC RAS
-
批准号:2882384
-
项目类别:
-
资助金额:$21.0万
-
财政年份:1992
-
负责人:JOHN J. COLICELLI
-
依托单位:
EFFECTORS AND REGULATORS OF NORMAL AND ONCOGENIC RAS
-
批准号:2008026
-
项目类别:
-
资助金额:$19.87万
-
财政年份:1992
-
负责人:JOHN J. COLICELLI
-
依托单位:
EFFECTORS AND REGULATORS OF NORMAL AND ONCOGENIC RAS
-
批准号:2667940
-
项目类别:
-
资助金额:$20.49万
-
财政年份:1992
-
负责人:JOHN J. COLICELLI
-
依托单位:
EFFECTORS AND REGULATORS OF NORMAL AND ONCOGENIC RAS
-
批准号:6164076
-
项目类别:
-
资助金额:$21.76万
-
财政年份:1992
-
负责人:JOHN J. COLICELLI
-
依托单位:
EFFECTORS AND REGULATORS OF NORMAL AND ONCOGENIC RAS
-
批准号:6644607
-
项目类别:
-
资助金额:$2.29万
-
财政年份:1992
-
负责人:JOHN J. COLICELLI
-
依托单位:
EFFECTORS AND REGULATORS OF NORMAL AND ONCOGENIC RAS
-
批准号:2097232
-
项目类别:
-
资助金额:$12.97万
-
财政年份:1992
-
负责人:JOHN J. COLICELLI
-
依托单位:
EFFECTORS AND REGULATORS OF NORMAL AND ONCOGENIC RAS
-
批准号:3200705
-
项目类别:
-
资助金额:$14.85万
-
财政年份:1992
-
负责人:JOHN J. COLICELLI
-
依托单位:
EFFECTORS AND REGULATORS OF NORMAL AND ONCOGENIC RAS
-
批准号:3200706
-
项目类别:
-
资助金额:$12.67万
-
财政年份:1992
-
负责人:JOHN J. COLICELLI
-
依托单位:
海外基金