MECHANISM OF ENZYME INHIBITION BY PHARMACOLOGICAL AGENTS

药理制剂抑制酶的机制

基本信息

  • 批准号:
    3418801
  • 负责人:
  • 金额:
    $ 6.06万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1993
  • 资助国家:
    美国
  • 起止时间:
    1993-08-01 至 1996-07-31
  • 项目状态:
    已结题

项目摘要

The objective of this proposal is to define the interaction between an enzyme and a highly specific competitive inhibitor. The enzyme being studied is a mammalian cAMP-specific isozyme of phosphodiesterase (PDE) that is strongly implicated in learning and memory functions. Inhibitors of this enzyme are pharmacologically useful in the treatment of some human pathologies. The work involves the use of a novel in vivo drug-resistance selection for mutants of a mammalian enzyme expressed in yeast. This reconstitution of a mammalian gene product into yeast cells results in a simplified system and a mechanism for the isolation of rare drug resistant mutants. The selection to be used is designed to isolate mammalian PDE mutants that are incapable of interacting with a competitive inhibitor (rolipram), but are not affected in substrate (cAMP) binding or hydrolysis. This work should lead to a better understanding of the nature of the interaction between a mammalian PDE enzyme and a pharmacologically useful drug. In addition, the methods employed in this research should prove useful in both the design and testing of more potent inhibitors of this enzyme. The methodology will also be extended to the study of other mammalian enzymes. These new techniques could provide an invaluable tool for the analysis of a wide variety of inhibitor-enzyme interactions.
此建议的目标是定义一个

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

JOHN J. COLICELLI其他文献

JOHN J. COLICELLI的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('JOHN J. COLICELLI', 18)}}的其他基金

A Novel Screen for Compounds that Outflank BCR-ABL Drug-Resistance
突破 BCR-ABL 耐药性的化合物的新筛选
  • 批准号:
    8067981
  • 财政年份:
    2009
  • 资助金额:
    $ 6.06万
  • 项目类别:
A Novel Screen for Compounds that Outflank BCR-ABL Drug-Resistance
突破 BCR-ABL 耐药性的化合物的新筛选
  • 批准号:
    7725809
  • 财政年份:
    2009
  • 资助金额:
    $ 6.06万
  • 项目类别:
Mechanism of RIN1 Signaling in Neuronal Plasticity
RIN1 信号传导在神经元可塑性中的机制
  • 批准号:
    6773149
  • 财政年份:
    2003
  • 资助金额:
    $ 6.06万
  • 项目类别:
Mechanism of RIN1 Signaling in Neuronal Plasticity
RIN1 信号传导在神经元可塑性中的机制
  • 批准号:
    6681142
  • 财政年份:
    2003
  • 资助金额:
    $ 6.06万
  • 项目类别:
Mechanism of RIN1 Signaling in Neuronal Plasticity
RIN1 信号传导在神经元可塑性中的机制
  • 批准号:
    7259412
  • 财政年份:
    2003
  • 资助金额:
    $ 6.06万
  • 项目类别:
Mechanism of RIN1 Signaling in Neuronal Plasticity
RIN1 信号传导在神经元可塑性中的机制
  • 批准号:
    7067644
  • 财政年份:
    2003
  • 资助金额:
    $ 6.06万
  • 项目类别:
Mechanism of RIN1 Signaling in Neuronal Plasticity
RIN1 信号传导在神经元可塑性中的机制
  • 批准号:
    6910730
  • 财政年份:
    2003
  • 资助金额:
    $ 6.06万
  • 项目类别:
MECHANISM OF ENZYME INHIBITION BY PHARMACOLOGICAL AGENTS
药理制剂抑制酶的机制
  • 批准号:
    2714522
  • 财政年份:
    1993
  • 资助金额:
    $ 6.06万
  • 项目类别:
MECHANISM OF ENZYME INHIBITION BY PHARMACOLOGICAL AGENTS
药理制剂抑制酶的机制
  • 批准号:
    2037678
  • 财政年份:
    1993
  • 资助金额:
    $ 6.06万
  • 项目类别:
MECHANISM OF ENZYME INHIBITION BY PHARMACOLOGICAL AGENTS
药理制剂抑制酶的机制
  • 批准号:
    2269863
  • 财政年份:
    1993
  • 资助金额:
    $ 6.06万
  • 项目类别:

相似海外基金

Extending the utility and durability of antifungal agents via innovative treatment regimens that minimise drug resistance
通过创新治疗方案最大限度地减少耐药性,延长抗真菌药物的效用和持久性
  • 批准号:
    MR/Y002164/1
  • 财政年份:
    2024
  • 资助金额:
    $ 6.06万
  • 项目类别:
    Research Grant
New approach based on enzyme stimulating of peptides for targeting drug resistance breast cancers
基于肽酶刺激的新方法用于靶向耐药性乳腺癌
  • 批准号:
    10713648
  • 财政年份:
    2023
  • 资助金额:
    $ 6.06万
  • 项目类别:
Functional and transcriptome analyses of protein kinases in Candida glabrata antifungal drug resistance
光滑念珠菌抗真菌药物耐药性中蛋白激酶的功能和转录组分析
  • 批准号:
    10643423
  • 财政年份:
    2023
  • 资助金额:
    $ 6.06万
  • 项目类别:
The role and mechanism of RNA m6A modification in the pathogenesis and drug-resistance of prostate cancer
RNA m6A修饰在前列腺癌发病及耐药中的作用及机制
  • 批准号:
    10638634
  • 财政年份:
    2023
  • 资助金额:
    $ 6.06万
  • 项目类别:
Mechanisms of HIV fitness and drug resistance inferred from high-resolution molecular dynamics and sequence co-variation models
从高分辨率分子动力学和序列共变模型推断出 HIV 适应性和耐药性的机制
  • 批准号:
    10750627
  • 财政年份:
    2023
  • 资助金额:
    $ 6.06万
  • 项目类别:
Reversing Drug Resistance in Tumors with Clickable Antibody Pairs
利用可点击的抗体对逆转肿瘤的耐药性
  • 批准号:
    10566266
  • 财政年份:
    2023
  • 资助金额:
    $ 6.06万
  • 项目类别:
Elucidation of prion strain-specific effect of antiprion drugs and drug resistance mechanisms
阐明抗朊病毒药物的朊病毒株特异性作用和耐药机制
  • 批准号:
    23H02828
  • 财政年份:
    2023
  • 资助金额:
    $ 6.06万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Mathematical Modeling of Drug Resistance Evolution and The Optimal Treatment Strategy in EGFR Mutated Lung Cancer
EGFR突变肺癌耐药演化的数学模型及最佳治疗策略
  • 批准号:
    22KJ0768
  • 财政年份:
    2023
  • 资助金额:
    $ 6.06万
  • 项目类别:
    Grant-in-Aid for JSPS Fellows
Targeting RAGE in tumor and TME to oppose inflammation and drug resistance in obesity associated ER+ breast cancer
靶向肿瘤和 TME 中的 RAGE,对抗肥胖相关 ER 乳腺癌的炎症和耐药性
  • 批准号:
    10734834
  • 财政年份:
    2023
  • 资助金额:
    $ 6.06万
  • 项目类别:
Targeting chromosomal insatiability for overcoming drug resistance
针对染色体不饱和性克服耐药性
  • 批准号:
    23K08200
  • 财政年份:
    2023
  • 资助金额:
    $ 6.06万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了