NOVEL MRI STUDY--NEURONAL LOSS IN TRANSGENIC MICE
新颖的 MRI 研究——转基因小鼠的神经元损失
基本信息
- 批准号:2002095
- 负责人:
- 金额:$ 7.25万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1996
- 资助国家:美国
- 起止时间:1996-09-30 至 1998-09-29
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Alzheimer's disease (AD) is an age-related illness that occurs in almost
50% of peopls over 80 years old. The hallmarks of AD include specific
abnormalities in brain structures. Particularly, biomedical magnetic
resonance imaging (MRI) has demonstrated a significant loss of gray matter
in AD patients. However, the exact mechanism of this cerebral atrophy in
AD remains unknown. This project will address this problem.
Our goal of understanding the structural changes in AD will be addressed by
combining expertise in three research areas; state-of-the-art microscopic
MRI, tissue segmentation, and mouse genetic engineering. The integration
of these techniques for animal modeling of Alzheimer's disease will promote
the understanding of the neuronal progression in this disease and lead to
early diagnosis. Based on the evidence that presence of the E4 allele of
apolipoprotein E. (APOE)) significantly correlates with the risk of an
individual developing AD, we hypothesize that MRI will show cerebral
atrophy in brains of transgenic mice carrying the human APOE4 allele. It
is expected that this will occur at a stage before behavioral analysis of
cognitive capabilities shows abnormalities. Four groups of mice will be
imaged using MRI. Three groups are the transgenic mouse lines carrying and
expressing human APOE, 2, 3, and 4 genes, respectively, and one group is
composed of nontransgenic age-matched controls. Each group will have
twenty mice. The imaging experiment will be repeated at one-month
intervals throughout the aging process. All MR images of mice brains will
be segmented into gray matter (GM) and white matter (WM) images by a fuzzy
classifier method. The mean and standard error of the GM and WM will be
calculated for each group. An ANOVA statistical analysis will be performed
on the images at each age to determine the GM and WM volume difference and
to assess the statistical significance for these four groups. The
relationship between changes in structure and age will be determined. The
results from this transgenic animal model study will provide a means to
characterize the development of Alzheimer's disease (neuronal destruction
and cognitive decline) which may result from the APOE allele interactions.
阿尔茨海默病(AD)是一种与年龄相关的疾病,几乎发生在
50%的人年龄在80岁以上。AD的特点包括特定的
大脑结构的异常。特别是生物医学磁学
磁共振成像(Mri)显示灰质显著丢失。
在AD患者中。然而,这种脑萎缩的确切机制是
广告仍不为人所知。这个项目将解决这个问题。
我们的目标是了解AD的结构变化,具体内容如下
结合三个研究领域的专业知识;最先进的显微技术
核磁共振、组织分割和小鼠基因工程。整合
这些阿尔茨海默病动物模型的技术将促进
对这种疾病中神经元进展的理解并导致
早期诊断。根据E4等位基因存在的证据,
载脂蛋白E(APOE))与高血压的风险显著相关
患有阿尔茨海默病的个体,我们假设核磁共振将显示大脑
携带人类载脂蛋白4等位基因的转基因小鼠的大脑萎缩。它
预计这将发生在行为分析之前的某个阶段
认知能力显示出异常。四组小鼠将被
用核磁共振成像。三组分别为携带和
分别表达人APOE、2、3和4基因,其中一组是
由年龄匹配的非转基因对照组组成。每组都将有
二十只老鼠。成像实验将在一个月内重复进行
在整个老化过程中的间隔时间。所有小鼠大脑的磁共振图像都将
通过模糊分割将图像分割为灰质(GM)和白质(WM)图像
分类器方法。GM和WM的平均误差和标准误差为
为每组计算的。将执行ANOVA统计分析
在每个年龄段的图像上确定GM和WM体积差和
评价四组间的统计学意义。这个
将确定结构变化与年龄之间的关系。这个
这项转基因动物模型研究的结果将提供一种手段
阿尔茨海默病的发展特征(神经元破坏
和认知能力下降),这可能是由载脂蛋白E等位基因相互作用造成的。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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JIA-HONG GAO的其他文献
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{{ truncateString('JIA-HONG GAO', 18)}}的其他基金
In Vivo Measurements of Physiologically-evoked Neuronal Currents
生理诱发神经元电流的体内测量
- 批准号:
8385726 - 财政年份:2012
- 资助金额:
$ 7.25万 - 项目类别:
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$ 7.25万 - 项目类别:
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$ 7.25万 - 项目类别:
CRCNS: Development & Optimization of Magnetic Source MRI
CRCNS:发展
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7208715 - 财政年份:2004
- 资助金额:
$ 7.25万 - 项目类别:
CRCNS: Development & Optimization of Magnetic Source MRI
CRCNS:发展
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7117398 - 财政年份:2004
- 资助金额:
$ 7.25万 - 项目类别:
CRCNS: Development & Optimization of Magnetic Source MRI
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PET Functional Brain Mapping : Intersubject Variabiliy
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- 批准号:
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- 批准号:
6500903 - 财政年份:2002
- 资助金额:
$ 7.25万 - 项目类别:
High Field (3 T) MRI Scanner at San Antonio, Texas
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