CANCER, LIPID, STRESS, AND THYROID RESEARCH

癌症、脂质、压力和甲状腺研究

• 批准号: 2167487
• 负责人: GUSTAV A OFOSU
• 金额: $ 44.88万
• 依托单位: DELAWARE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-07-01 至 1997-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2167487
• 关键词: CANCER; LIPID; STRESS; THYROID; RESEARCH

The proposed program is designed to motivate minority faculty members to engage in biomedical research, provide opportunities for minority students to gain biomedical research experience, and interest students in pursuing research careers in the biomedical sciences. To this end the subprojects are: 1) MOLECULAR GENOTYPING OF BLACKS WITH GRAVES' DISEASE. Restriction fragment length polymorphisms (RFLPs) and human leukocyte antigens (HLA) will be determined in blacks with Graves' Disease, their families, diabetics and normal controls. Southern blotting hybridization method will be used and DR alpha, DR beta, DQ beta and DP beta inserts used in nick translation will be obtained from cDNA clones. 2) LIPOLYTIC ENZYMES AND THEIR SUBSTRATES OF HEART, LUNG, KIDNEY AND BRAIN. The goal of this program is to contribute to the understanding of the in vivo purposes of each (and collectively) of the wide variety of endogenous ester, ether and sphingo-lipids characterizing the kidney, heart, lung and brain by an in vitro examination of their endogenous lipolytic capabilities. An elaborate and definitive system of chromatographic analysis of their lipids will provide the data. 3) PERSONALITY FACTORS IN THE DETECTION AND CONTROL OF STRESS-RELATED PHYSIOLOGICAL VARIABLES. This project involves investigation of personality factors related to the individual's ability to detect and voluntarily control physiological variables related to stress induced or psychosomatic disorders. 4) NON-NEPHROTOXIC PLATINUM COORDINATION COMPLEXES IN COMBINATION CHEMOTHERAPY OF CANCER CELLS. The project includes ultrastructural and histochemical studies of the combined application of iproplatin, carboplatin and platinum-thymine on ascites sarcoma. Dose-limiting side effects in combination therapy will be monitored. The combined drug effects on plasma membrane phosphatases will be investigated. 5) HETEROPOLYFLUOROXOMETALATES AS ANTIVIRAL AGENTS. The principal objectives of the proposal study are to prepare, characterize, and explore the antiviral activity of heteropolyfluoroxometalates anions containing Mo, W, and possibly Ru, and Re as addenda atoms. The antiviral activity will be explored by (1) in vitro screening as antiviral agents, (2) testing samples as part of the anti-HIV screening process, and (3) study the inhibitory effect of the compound on some vital viral enzymes. 6) THE SOLUTION, PURIFICATION, AND SYNTHESIS OF TOXIC AMINO ACIDS HYPOGLYCINE A AND HYPOGLYCINE B. This project involves investigation of two amino acids that could eventually serve as compounds to lower blood sugar. A unique isolation procedure will be used to purify the substances.

该计划旨在激励少数民族教师， 从事生物医学研究，为少数民族学生提供机会 获得生物医学研究经验，并感兴趣的学生在追求 在生物医学科学的研究生涯。 为此， 为： 1)黑人Graves病的分子基因分型 限制 片段长度多态性（RFLPs）与人类白细胞抗原（HLA） 将在患有格雷夫斯病的黑人、他们的家人、 糖尿病患者和正常对照组。 Southern印迹杂交方法将 使用DR α、DR β、DQ β和DP β衬垫 翻译将从cDNA克隆获得。 2)心、肺、肾和脑的脂解酶及其底物。 该计划的目标是有助于了解在 体内目的的每一个（和集体）的各种内源性 酯，醚和鞘脂表征肾脏，心脏，肺和 通过对它们的内源性脂解的体外检查， 能力的 一个精细而明确的色谱系统， 分析他们的脂质将提供数据。 3)应激反应检测与控制中的个性因素 生理变量。 该项目涉及调查 与个人检测能力相关的人格因素， 自愿控制与应激诱导或 身心障碍 4)非肾毒性铂配合物的组合 癌细胞的化学疗法。 该项目包括超微结构和 联合应用异丙铂的组织化学研究， 卡铂和铂-胸腺嘧啶治疗腹水肉瘤。 剂量限制性副 将监测联合治疗的效果。 组合药物 将研究对质膜磷酸酶的影响。 5)作为抗病毒剂的杂多氟氧金属酸盐. 校长 提案研究的目标是准备、描述和探索 含钼的氟代杂多酸盐阴离子的抗病毒活性， W和可能的Ru和Re作为附加原子。 抗病毒活性将 通过（1）体外筛选作为抗病毒剂，（2）测试 样本作为抗艾滋病毒筛查过程的一部分，以及（3）研究 该化合物对某些重要病毒酶的抑制作用。 6)有毒氨基酸的溶解、纯化和合成 次甘氨酸A和次甘氨酸B。 该项目涉及调查 这两种氨基酸最终可以作为化合物降低血液 糖 将使用独特的分离程序纯化物质。