MICROBIAL RISK FACTORS ASSOCIATED WITH BABY BOTTLE CARIE

婴儿奶瓶蛀牙（BBTD）仍然是一个主要原因， 牙科病理学使人衰弱，费用高昂，影响到6%的儿童， 3岁虽然不适当的饮食习惯是最明显的风险， 家族性接种致龋微生物植物群， 这是一个额外的贡献因素。以前的研究者 总变形链球菌（MS）计数与BBTD严重程度相关，但 还没有人完成对相关菌株的详细描述， 与这种疾病。我们假设BBTD是由细胞增殖引起的， 变形链球菌的特定亚群， 可以从孩子的父母和/或兄弟姐妹那里获得。当转移 对于一个有不适当喂养习惯的孩子来说， 扩散并导致疾病。本研究旨在：（1）利用DNA 指纹分析和核糖体分型来确定一个 以前从发展中国家分离出的大量MS菌株 龋齿病变和对照部位，以确定是否有 特定亚型的S.与BBTD相关的μ tan，（2）评估 存在特定的S.儿童口腔中的变形菌 与BBTD及其父母和同胞，和（3）比较MS相关 在不同的种族群体中（美洲原住民，非洲裔美国人， 高加索人）。这项研究的长期目标是确定亚- 类型S.可能与BBTD相关的变异体。这些信息 然后可以用来识别和跟踪特定的菌株， 有效预防和治疗BBTD的策略。这些 策略可以包括在疾病发作之前对患者进行治疗， 为有风险的家庭提供微生物治疗，并可能预防 通过被动免疫接种治疗患者， 适当的抗体。

Baby bottle tooth decay (BBTD) continues to be a major cause of debilitating and costly dental pathology, affecting 6% of children under 3 years old. While inappropriate feeding habits are the most apparent risk factor, familial inoculation with a cariogenic microbial flora has been suggested as an additional contributing factor. Previous investigators have related total mutans streptococci (MS) counts to BBTD severity, but no one has completed a detailed characterization of the strains associated with this disease. We hypothesize that BBTD results from the proliferation of specific sub populations of Streptococcus mutans and that such strains may be obtained from the child's parents and/or siblings. When transferred to a child with inappropriate feeding habits, such strains may then proliferate and cause disease. This research seeks: (1) to utilize DNA fingerprinting and ribotyping to determine the genetic diversity of a large number of strains of MS previously isolated from developing nursing caries lesions and control sites in order to determine if there are specific subtypes of S. mu tans associated with BBTD, (2) to evaluate the presence of specific strains of S. mutans in the oral cavity of children with BBTD and their parents and sibs, and (3) to compare the MS associated with BBTD in different ethnic groups (Native American, African-American, Caucasian). The long-term goal of this research is to identify the sub- types of S. mutans which may be associated with BBTD. This information could then be used to identify and track specific strains for development of effective preventive and therapeutic strategies for BBTD. These strategies may include patient therapy prior to onset of disease, microbial therapy for families at risk and possibly prevention of recolonization of treated patients by passive immunization with appropriate antibodies.