DROSOPHILA TRNA 5' AND 3' END PROCESSING

描述(改编自申请人的摘要)：调查员 建议研究细胞内tRNA的5‘和3’加工要求 果蝇黑腹果蝇。5‘末端所需的两个RNaseP 以及3‘-tRNase的分离和性质 大分子组成。由于RNaseP通常具有RNA 任何与果蝇RNaseP互配的RNA都将被测序 为了与与之相关的RNA进行系统发育比较 其他物种的核糖核酸酶P。 将特定部位的突变引入tRNA进行分析 对底物识别和催化的要求。三个问题 将在这些研究中涉及：1.)TRNA的元件是如何 核糖核酸酶P和3‘-tRNase识别的结构不同？ 2.)这两种酶是识别特定的序列，还是只识别结构？ 和3.)第三级接触在多大程度上(例如，D和 T环)有助于底物识别？

DESCRIPTION (Adapted from the applicant's abstract): The investigator proposes to study requirement for 5' and 3' processing of tRNA in cells of Drosophila melanogaster. Both RNase P, required for 5' end processing, and 3'-tRNase will be isolated and characterized as to their macromolecular composition. Since RNase P generally has an RNA component, any RNA copurifying with Drosophila RNase P will be sequenced in order to make phylogenetic comparisons with RNAs associated with RNase P in other species. Site specific mutations will be introduced into tRNA to analyze requirements for substrate recognition and catalysis. Three questions will be addressed in these studies: 1.) How do the elements of tRNA structure recognized by RNase P and 3'-tRNase differ from each other? 2.) Does either enzyme recognize a specific sequence, or only structure? and 3.) To what extent do tertiary contacts (e.g., between the D and T loops) contribute to substrate recognition?