PHAGE EXPRESSION LIBRARIES FOR ANALYSIS OF MALARIA PARAS

用于分析副疟疾的噬菌体表达文库

• 批准号: 2293327
• 负责人: ALI A SULTAN
• 金额: $ 2.5万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-30 至 1997-08-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2293327
• 关键词: PHAGE; EXPRESSION; LIBRARIES; ANALYSIS; MALARIA

Target cell specificity for the invasion of human liver hepatocytes by malaria sporozites is conferred by a receptor-ligand interaction between hepatocytes plasma membrane heparin sulfate proteoglycans (HSPG) and an evolutionarily conserved region in the carboxy terminus of the malaria sporozoite coat protein, the circumsporozoite (CS) protein. The detailed structural requirements for this recognition are however, not fully understood and it may be possible to construct sequences or structural mimics of the conserved Region II-plus which bind the receptor with higher affinity than the native sequence. It is proposed to select such 'mimeotopes' from bacteriophage display libraries for use in experiments on inhibition of malarial infection in vivo and in vitro by blocking the recognition of sporozoite CS by the HSPG hepatocyte receptors.

靶细胞特异性可用于侵袭人肝肝细胞 疟疾孢子是由受体 - 配体相互作用赋予的 肝细胞质膜硫酸盐蛋白聚糖（HSPG）和A 疟疾的羧基末端的进化保守区域 Sporozoite涂层蛋白，外孢菌岩（CS）蛋白。详细 但是，这种识别的结构要求尚未完全 理解，可能可以构建序列或结构 模仿保守区域II-Plus，该区域结合受体与 比天然序列更高的亲和力。建议选择这样的 噬菌体显示库中的“ Mimeotopes”用于实验 通过阻断体内和体外疟疾感染的抑制 HSPG肝细胞受体对Sporozoite CS的识别。