PROTEIN/DNA INTERACTIONS IN THE YEAST GENOME

描述：（申请人摘要）酵母基因组序列现在是已知的， 这是一项重大的技术成就， 研究最简单的真核生物的细胞生物学。 知识 调节蛋白和结构蛋白与DNA在体内的相互作用， 酵母DNA序列对其功能的应用很重要。 这 我们的建议涉及如何实现这一目标。 它建立在新兴的 DNA甲基转移酶在绘制蛋白质-DNA相互作用中的应用 活细胞通过开发方法，将允许分辨率（<10碱基 pairs）研究了S.酿酒酵母 基因组 它描述的方法，将允许定量评估 蛋白质-DNA相互作用的自动化技术， 用于酵母基因组的大区域，并可输出到较大的真核生物。 为了说明该方法的实用性，我们将绘制染色质 组织大约一半的染色体III。 比较 结构与经典方法所确定的。 许多其他 实验室对染色质结构及其作用都非常感兴趣 DNA功能和调节蛋白与DNA的相互作用。 到 为了促进这些科学家的研究，我们提出了一项服务， 为酵母基因组的其他区域提供染色质图谱数据， 兴趣

DESCRIPTION: (Applicant's abstract) The yeast genome sequence is now known, a major technical accomplishment that offer unprecedented opportunities for study of the cell biology of the simplest eukaryote. Knowledge of the interactions of regulatory and structural proteins with DNA in vivo is important for application-of the yeast DNA sequence to its function. This proposal addresses how to accomplish that goal. It builds on emerging applications of DNA methyltransferases to map protein-DNA interactions in living cells by developing methodology that will allow resolution (<l0 base pairs) study of interactions over extended regions of the S. cerevisiae genome. It describes methodology which will allow quantitative assessment of protein-DNA interactions with automated techniques that are appropriate for large regions of the yeast genome and exportable to larger eukaryotes. To illustrate the utility of the approach, we will map the chromatin organization of about half of chromosome III. Comparing portions of the structure with that determined by classical methods. Many other laboratories are highly interested both in chromatin structure and its role in DNA function and in interactions of regulatory proteins with DNA. To facilitate the research of these scientists, we propose a service which will provide chromatin mapping data for other regions of the yeast genome of interest.