CHEMISTRY AND BIOLOGY OF HUMAN LYMPHOCYTES

人类淋巴细胞的化学和生物学

• 批准号: 2007714
• 负责人: JACK L STROMINGER
• 金额: $ 225.06万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-30 至 2001-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2007714
• 关键词: B lymphocyte; CD4 molecule; CD8 molecule; Epstein Barr virus; MHC class I antigen; MHC class II antigen; T cell receptor; blocking antibody; chromatin; crystallization; gene expression; genetic enhancer element; genetic promoter element; genetic regulation; human subject; integrins; laboratory mouse; leukocyte activation /transformation; lymphocyte proliferation; natural killer cells; protein structure function; protein tyrosine kinase; tissue /cell culture; transcription factor; tumor necrosis factor alpha

This application proposes studies in a variety of areas relating to the chemistry and biology of human lymphocytes. The problems being studied involve the molecular basis of immune recognition, including the recognition of tumor cells, as well as factors which govern and regulate proliferation of human lymphocytes. The techniques being employed include protein chemistry and crystallography, molecular genetics, cellular immunology and virology. Specific areas of investigation are: 1) protein chemistry and crystallography of and the relationship of structure to function in class I MHC molecules and their bound peptides, 2) similar studies of class II MHC molecules, 3) the interaction of CD4 with the class Il MHC molecule through its D3 and D4 domains and with the protein tyrosine kinase p56(lck) through its intracytoplasmic region, 5) protein chemistry of T cell receptors and the mechanisms of development of T cells, 6) the role of HLA-C in NK allorecognition and other studies of NK cells, 7) the mechanism of transformation of B and T cells by Epstein Barr Virus, and 8) the regulation of expression and function of tumor necrosis factor alpha. They relate to tumor immunology and tumor cell proliferation in a variety of ways.

该申请提出了与 人类淋巴细胞的化学和生物学。正在研究的问题 涉及免疫识别的分子基础，包括 识别肿瘤细胞，以及控制和调节 人淋巴细胞的增殖。所采用的技术包括 蛋白质化学和晶体学，分子遗传学，细胞 免疫学和病毒学。具体研究领域有：1）蛋白质 化学和晶体学以及结构与 在I类MHC分子及其结合肽中的功能，2）类似于 II类MHC分子的研究，3）CD 4与 II类MHC分子通过其D3和D4结构域与蛋白质结合 酪氨酸激酶p56（lck）通过其胞质内区域，5）蛋白 T细胞受体的化学和T细胞发育的机制 6）HLA-C在NK细胞同种异体识别中的作用及NK细胞的其他研究 7）Epstein巴尔转化B和T细胞的机制 8）肿瘤坏死因子表达和功能的调控 α因子它们与肿瘤免疫学和肿瘤细胞增殖有关 以不同的方式。