REGULATION OF THE RAT VIP RECEPTOR GENE

研究：血管活性肠肽的广泛生物学功能是由 它的受体。在肺中，VIP作为一种有效的平滑肌松弛剂和 它还可能调节肺癌的增殖。这样做的长期目标是 建议确定调节VIP受体的分子机制 在发育过程中和疾病中的表达。具体目标是 分离并鉴定大鼠血管活性肠肽受体基因(2)。身份 血管活性肠肽受体转录的调控元件和因子 正常和癌变肺细胞的转染及DNA-蛋白结合 分析(3)。血管活性肠肽受体表达调控的研究 通过原位杂交和同源重组获得 产生缺乏功能性VIP受体的小鼠。这些研究将 为VIP受体调节的生理学提供了重要的见解 对这种无处不在的荷尔蒙起作用。这将导致增强 了解VIP反应性恶性肿瘤的发病机制。 应聘者：林培博士，医学博士，博士，有很强的 分子生物学。在加州大学洛杉矶分校早期的博士后培训期间，她还 掌握蛋白质化学方面的技能。这将使她能够有效地 完成并解释本提案中的实验。贝医生是 致力于应用分子生物学技术回答问题 疾病病理生理学教授，开始了他的学术生涯 医药。作为一名独立的教员，她得到了院系的支持 在颁奖过程中和颁奖结束前都是会员。 环境：申请者将在设备齐全的 赞助商的实验室，由NIDDK计划项目资助 肿瘤的内分泌方面。因此，贝博士的建议将遇到 集团的总体目标。她将拥有所有核心的完全访问权限 戴维斯研究大楼内提供的设施包括分子 寡核苷酸合成，转基因小鼠和子宫。有每周一次的 正在进行研究的实验室会议、部门研究研讨会 和访问科学家(生长因子、转录、基因调控) 提供持续接触基础和临床研究进展的机会。在……里面 此外，贝博士将享受与专家的持续接触 锡达斯-西奈大学的内分泌学院以及相关的生产团体 分子遗传学、膜生物学、移植生物学、病毒学 和蛋白质化学。

Research: The broad spectrum of VIP biological functions are mediated by its receptor. In the lung, VIP acts as a potent smooth muscle relaxant and it may also regulate lung cancer proliferation. The long-term goal of this proposal is to determine molecular mechanisms that regulate VIP receptor expression during development and in disease. The specific aims are to isolate and characterized the rat VIP receptor gene (2). Identity regulatory elements and factors that control VIP receptor transcription in both normal and cancerous lung cells transfection and DNA-protein binding assays (3). Study the regulation of VIP receptor expression during development by in situ hybridization and by homologous recombination to generate mice that lack functional VIP receptors. These studies will provide important insight into the physiology of VIP receptor regulation and function to this ubiquitous hormone. This will lead to enhanced understanding mechanisms for VIP-responsive malignancies. Candidate: Dr. Lin Pei, M.D., Ph.D., has a strong background training in molecular biology. During early postdoctoral training at UCLA, she also acquired skills in protein chemistry. This will enable her to effectively perform and interpret the experiments in this proposal. Dr. Pei is committed to applying molecular biological techniques to answer questions of disease pathophysiology and is embarked on a career in academic medicine. She is assured of Departmental support as an independent faculty member both during and by the end of the award. Environment: The applicant will perform the research in the well-equipped laboratory of the sponsor which is funded as a NIDDK Program Project in endocrine aspects of neoplasia. As such, Dr. Pei's proposal will meet with the overall goals of the group. She will have full access to all core facilities available in the Davis Research Building including molecular oligonucleotide synthesis, transgenic mouse and vivarium. There are weekly research-in-progress laboratory meeting, departmental research seminars and visiting scientists (growth factors, transcription, gene regulator) providing continued exposure to basic and clinical research advances. In addition, Dr. Pei will enjoy the continued exposure to the expert endocrine faculty at Cedars-Sinai, as well as associated productive groups in molecular genetics, membrane biology, transplantation biology, virology and protein chemistry.