REGULATION OF THE RAT VIP RECEPTOR GENE

大鼠 VIP 受体基因的调控

• 批准号: 2904920
• 负责人: LIN PEI
• 金额: $ 10.87万
• 依托单位: CEDARS-SINAI MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-30 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2904920
• 关键词: DNA binding protein; RNase protection assay; animal genetic material tag; biological signal transduction; developmental genetics; embryo /fetus; embryonic stem cell; gene targeting; genetic regulation; genetically modified animals; histogenesis; hormone receptor; immunocytochemistry; in situ hybridization; laboratory mouse; laboratory rat; lung; lung neoplasms; mammalian embryology; neuropeptide receptor; point mutation; receptor expression; site directed mutagenesis; transcription factor; vasoactive intestinal peptide

Research: The broad spectrum of VIP biological functions are mediated by its receptor. In the lung, VIP acts as a potent smooth muscle relaxant and it may also regulate lung cancer proliferation. The long-term goal of this proposal is to determine molecular mechanisms that regulate VIP receptor expression during development and in disease. The specific aims are to isolate and characterized the rat VIP receptor gene (2). Identity regulatory elements and factors that control VIP receptor transcription in both normal and cancerous lung cells transfection and DNA-protein binding assays (3). Study the regulation of VIP receptor expression during development by in situ hybridization and by homologous recombination to generate mice that lack functional VIP receptors. These studies will provide important insight into the physiology of VIP receptor regulation and function to this ubiquitous hormone. This will lead to enhanced understanding mechanisms for VIP-responsive malignancies. Candidate: Dr. Lin Pei, M.D., Ph.D., has a strong background training in molecular biology. During early postdoctoral training at UCLA, she also acquired skills in protein chemistry. This will enable her to effectively perform and interpret the experiments in this proposal. Dr. Pei is committed to applying molecular biological techniques to answer questions of disease pathophysiology and is embarked on a career in academic medicine. She is assured of Departmental support as an independent faculty member both during and by the end of the award. Environment: The applicant will perform the research in the well-equipped laboratory of the sponsor which is funded as a NIDDK Program Project in endocrine aspects of neoplasia. As such, Dr. Pei's proposal will meet with the overall goals of the group. She will have full access to all core facilities available in the Davis Research Building including molecular oligonucleotide synthesis, transgenic mouse and vivarium. There are weekly research-in-progress laboratory meeting, departmental research seminars and visiting scientists (growth factors, transcription, gene regulator) providing continued exposure to basic and clinical research advances. In addition, Dr. Pei will enjoy the continued exposure to the expert endocrine faculty at Cedars-Sinai, as well as associated productive groups in molecular genetics, membrane biology, transplantation biology, virology and protein chemistry.

研究：广泛的VIP生物学功能是由

项目成果

Identification of a negative glucocorticoid response element in the rat type 1 vasoactive intestinal polypeptide receptor gene.

大鼠 1 型血管活性肠多肽受体基因中负性糖皮质激素反应元件的鉴定。

• 发表时间: 1996
• 期刊: The Journal of biological chemistry
• 作者: Pei,L

Molecular cloning of a novel transcriptional repressor protein of the rat type 1 vasoactive intestinal peptide receptor gene.

大鼠 1 型血管活性肠肽受体基因的新型转录抑制蛋白的分子克隆。

• DOI: 10.1074/jbc.273.31.19902
• 发表时间: 1998
• 期刊: The Journal of biological chemistry
• 作者: Pei,L

Genomic organization and identification of an enhancer element containing binding sites for multiple proteins in rat pituitary tumor-transforming gene.

大鼠垂体肿瘤转化基因中含有多种蛋白质结合位点的增强子元件的基因组组织和鉴定。

• DOI: 10.1074/jbc.273.9.5219
• 发表时间: 1998
• 期刊: The Journal of biological chemistry
• 作者: Pei,L

Pituitary tumor-transforming gene protein associates with ribosomal protein S10 and a novel human homologue of DnaJ in testicular cells.

垂体肿瘤转化基因蛋白与核糖体蛋白 S10 和睾丸细胞中 DnaJ 的新型人类同源物相关。

• DOI: 10.1074/jbc.274.5.3151
• 发表时间: 1999
• 期刊: The Journal of biological chemistry
• 作者: Pei,L

Isolation and characterization of a pituitary tumor-transforming gene (PTTG).

• DOI: 10.1210/mend.11.4.9911
• 发表时间: 1997-04
• 期刊: Molecular endocrinology
• 作者: L. Pei;S. Melmed