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INTRACELLULAR PROCESSING OF RAT KIDNEY RENIN

大鼠肾肾素的细胞内加工

基本信息

批准号：
2430166
负责人：
JULIE R. INGELFINGER
金额：
$ 8.99万
依托单位：
MASSACHUSETTS GENERAL HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
1993
资助国家：
美国
起止时间：
1993-06-01 至 2002-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2430166
关键词：
arterioles endocrinology enzyme activity glycosylation intracellular transport kidney cell kidney function laboratory rabbit laboratory rat protein isoforms renin secretion tissue /cell culture vasomotion western blottings

项目摘要

The physiologic importance of the renin-angiotensin system (RAS) in maintaining cardiovascular homeostasis is undisputed. Recent observations have expanded the traditional views of the morphology and function of renin-secreting cells. A) Two distinct renal cell types, epithelioid juxtaglomerular cells (JG cells) and distal afferent arteriolar smooth muscle cells, are known to contain and secrete renin. B) The renin angiotensin system has local and systemic actions in controlling cardiovascular homeostasis. C) Renin isoforms with differing metabolic half lives have been identified. The working hypothesis is that JG cells and distal afferent arteriolar cells contain and secrete different renin isoforms. Through these differences the two cell types may contribute in distinct ways to regulation of vascular homeostasis. The long term goal of this proposal is to define the secretory patterns and renin isoforms of unstimulated and stimulated renin release in rat kidney JG cells and distal afferent arteriolar cells. In this proposal, Dr. Norling will apply cell biology techniques of pulse chase analysis of secretion for studying renin secretion in two renin- containing cell types from rat kidney. Renin isoforms from these cells will be characterized and compared. From these experiments it is expected that Dr. Norling will find that JG cells and distal afferent arteriolar cells have renin processing capabilities which are cell-type specific. This information will help to expand the understanding of RAS regulation of vascular tone.

肾素-血管紧张素系统（RAS）在糖尿病中的生理学意义维持心血管内稳态是无可争议的。最近观察扩展了形态学的传统观点，肾素分泌细胞的功能。 A）两种不同的肾细胞类型，上皮样肾小球细胞（JG细胞）和远端传入纤维已知小动脉平滑肌细胞含有并分泌肾素。 B）肾素血管紧张素系统具有局部和全身作用，控制心血管的体内平衡 C）具有不同的代谢半衰期已经确定。工作假设是 JG细胞和远端传入小动脉细胞含有并分泌不同的肾素异构体。通过这些差异，两种细胞类型可能以不同的方式调节血管内稳态。这项建议的长期目标是确定分泌模式大鼠非刺激性和刺激性肾素释放及其亚型肾JG细胞和远端传入小动脉细胞。在这项提案中，诺林博士将应用细胞生物学技术的脉冲用分泌物追踪分析法研究两种肾素-血管紧张素转换酶的分泌，包含来自大鼠肾脏的细胞类型。来自这些细胞的Renin亚型将被描述和比较。从这些实验来看，我希望Norling博士能发现JG细胞和远端传入神经小动脉细胞具有细胞型的肾素处理能力，特定. 这些信息将有助于扩大对RAS的理解调节血管张力。