NEUTRALIZABLE EPITOPES OF CHLAMYDIA TRACHOMATIS

沙眼衣原体的可中和表位

• 批准号: 2003631
• 负责人: ELLENA Marie PETERSON
• 金额: $ 18.57万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-07-01 至 1998-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2003631
• 关键词: Chlamydia trachomatis; active immunization; antibody neutralization test; bacterial antigens; complement; crosslink; drug administration routes; drug delivery systems; enzyme linked immunosorbent assay; epitope mapping; host organism interaction; laboratory mouse; membrane proteins; model design /development; monoclonal antibody; neutralizing antibody; passive immunization; protein structure function; serum; synthetic peptide

The overall goal of this proposal is to determine whether peptides corresponding to neutralizable contiguous epitopes of the major outer membrane protein (MOMP) of Chlamydia trachomatis are able to confer protection from an infection with this organism. This pathogen is the leading cause of sexually transmitted disease in the Western world and has been implicated as a major contributing factor to infertility. It is also the leading cause of preventable blindness in underdeveloped countries. Due to the morbidity associated with this organism, attempts have been made to vaccinate with the intact organism. From vaccine trials it was concluded that the host immune response contributed to the damaging sequelae associated with a chlamydial infection. More recently in an effort to both circumvent the hypersensitivity reaction as well as increase the number of serovars represented in a vaccine, subunit vaccines have been proposed as a possible solution. Although we realize that both protective B and T cell epitopes will eventually need to be incorporated into an effective vaccine, in this proposal we will focus on epitopes that we have identified and characterized as contiguous and neutralizable to determine whether peptides representing these epitopes can afford protection to the host. We will first focus on serovar E since it is the most common genital isolate of C. trachomatis. Peptides representing neutralizable epitopes will be tested alone, in combination and as a colinear peptide construct for their ability to elicit a protective immune response. The colinear peptides will be used to establish the optimal peptide construction, immunization route, dose, delivery system and if necessary, adjuvant that will elicit a strong mucosal response. Paralleling these efforts we will be working with a murine model of a chlamydial genital infection in order to establish a reproducible model that will be suitable for testing the ability of the immunization parameters established with the peptide constructs to protect or attenuate from a chlamydial infection and the sequelae of infertility. Information gained from this approach should bring us closer to answering the question as to the role of neutralizing antibodies in chlamydial infections.

本提案的总体目标是确定肽是否

项目成果

Immunization with a peptide corresponding to chlamydial heat shock protein 60 increases the humoral immune response in C3H mice to a peptide representing variable domain 4 of the major outer membrane protein of Chlamydia trachomatis.

用对应于衣原体热休克蛋白60的肽进行免疫增强了C3H小鼠中对代表沙眼衣原体主要外膜蛋白可变结构域4的肽的体液免疫应答。

• DOI: 10.1128/cdli.6.3.356-363.1999
• 发表时间: 1999
• 期刊: Clinical and diagnostic laboratory immunology
• 作者: Motin,VL;delaMaza,LM;Peterson,EM
• 通讯作者: Peterson,EM

Characterization of a neutralizing monoclonal antibody directed at the lipopolysaccharide of Chlamydia pneumoniae.

针对肺炎衣原体脂多糖的中和单克隆抗体的表征。

• DOI: 10.1128/iai.66.8.3848-3855.1998
• 发表时间: 1998
• 期刊: Infection and immunity
• 影响因子: 3.1
• 作者: Peterson,EM;delaMaza,LM;Brade,L;Brade,H
• 通讯作者: Brade,H

Effect of immunoglobulin G isotype on the infectivity of Chlamydia trachomatis in a mouse model of intravaginal infection.

免疫球蛋白 G 同种型对小鼠阴道内感染模型中沙眼衣原体感染性的影响。

• DOI: 10.1128/iai.65.7.2693-2699.1997
• 发表时间: 1997
• 期刊: Infection and immunity
• 影响因子: 3.1
• 作者: Peterson,EM;Cheng,X;Motin,VL;delaMaza,LM
• 通讯作者: delaMaza,LM

Characterization of a neutralizing monoclonal antibody directed at variable domain I of the major outer membrane protein of Chlamydia trachomatis C-complex serovars.

针对沙眼衣原体 C 复合物血清型主要外膜蛋白可变结构域 I 的中和单克隆抗体的表征。

• DOI: 10.1128/iai.61.4.1365-1370.1993
• 发表时间: 1993
• 期刊: Infection and immunity
• 影响因子: 3.1
• 作者: Qu,Z;Cheng,X;delaMaza,LM;Peterson,EM
• 通讯作者: Peterson,EM

The effect of orientation within a chimeric peptide on the immunogenicity of Chlamydia trachomatis epitopes.

嵌合肽内的方向对沙眼衣原体表位免疫原性的影响。

• DOI: 10.1016/0161-5890(95)00157-3
• 发表时间: 1996
• 期刊: Molecular immunology
• 影响因子: 3.6
• 作者: Peterson,EM;Cheng,X;Qu,Z;delaMaza,LM
• 通讯作者: delaMaza,LM