Brillouin microscopy for high-speed imaging of rigidity within cells

用于细胞内刚性高速成像的布里渊显微镜

• 批准号: DE190100641
• 负责人: Dr Michael Taylor
• 金额: $ 29.64万
• 依托单位: The University of Queensland
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Early Career Researcher Award
• 财政年份: 2019
• 资助国家: 澳大利亚
• 起止时间: 2019-04-01 至 2022-07-30
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DE190100641
• 关键词: Brillouin; microscopy; speed; imaging; rigidity

This project aims to improve the sensitivity and speed of Brillouin microscopes. Brillouin microscopes use light to measure the stiffness of samples in 3D without requiring physical access, allowing their use in inaccessible locations such as the interior of cells or within intact tissue. However, Brillouin microscopes are too slow to be used in most research. This project introduces a new approach based on different optical physics that is expected to enable faster and more precise imaging. The microscope will be used to study the movement of amoeba, where it is expected to reveal the controlled stiffening and fluidising of the different regions of protoplasm believed to underlie the cell mobility.

该项目旨在提高布里渊显微镜的灵敏度和速度。布里渊显微镜使用光来测量3D样品的硬度，而不需要物理访问，允许它们在不可访问的位置使用，例如细胞内部或完整组织内。然而，布里渊显微镜太慢，无法用于大多数研究。该项目引入了一种基于不同光学物理的新方法，有望实现更快、更精确的成像。该显微镜将用于研究变形虫的运动，预计将揭示原生质不同区域的受控硬化和流动，这些区域被认为是细胞运动的基础。