DIAGNOSTIC OPTIMAL IMAGING OF PERIODONTAL TISSUES

光学相干断层扫描（OCT）基于以下来检测组织界面： 它们对光的不同反射。这些技术是非侵入性的 并采用具有相当深度的光波长， 组织中的渗透，但没有已知的有害生物效应。 本提案中描述的研究将确定这项技术是否可以 可作为牙周病诊断的一种新的影像学工具。我们会适应的 现有的激光设备，可通过我们在劳伦斯合作 利弗莫尔实验室，以测试这一假设。我们的初步数据 证明了足够的光穿透牙龈组织来记录 有意义的光信号此外，在眼科应用中， OCT提供比传统诊断技术更高的分辨率。 光学成像将有许多优势，现有的牙周 诊断程序。OCT将产生二维或三维图像 描绘了囊袋的拓扑结构和结缔组织附着， 可用于评估硬组织和软组织之间的关系。这些 可视记录可以作为患者记录的一部分归档。错误 与检查者在囊袋探测测量中的可变性相关， 淘汰根分叉受累与早期骨丢失相关 附着可以被检测、快速记录并与随后的附着进行比较。 治疗后的阅读开窗和裂开，通常不 在手术前确诊，也可以检测到。患者对此的接受程度 技术将远远大于大多数牙科手术。oct是 完全非侵入性，可重复进行，以监测进展 对病人没有任何危险。OCT是无痛的，快速的， 可以很容易地纳入常规牙科检查。 这项技术也将成为一个强大的研究工具。oct可以 可能用于区分活动和非活动 牙周病胶原纤维的取向之前，期间 治疗后可以反复评估，以评估进展 牙周病治疗此外，这些光学技术可以 光谱检测，定量和监测小浓度的 特定分子这种潜在的应用将允许快速，非- 牙周袋内多种分子的侵入性测量。 该提案的具体目标是： 1.调整现有激光设备和技术，以证明OCT 可用于牙周诊断。 2.将OCT的诊断结果与获得的测量结果进行比较 用组织学方法。 3.建立一个原型的临床牙周OCT成像系统， 成本是负担得起的临床牙科实践。

Optical coherence tomography (OCT) detects tissue interfaces based on their differential reflection of light. These techniques are non-invasive and employ wavelengths of light that have considerable depth of penetration in tissue but no known detrimental biological effects. Studies described in this proposal will determine if this technology can be used as a new imaging tool for periodontal diagnosis. We will adapt existing laser equipment, available through out collaboration at Lawrence Livermore Laboratories, to test this hypothesis. Our preliminary data demonstrated that sufficient light penetrates gingival tissue to record a meaningful optical signal. Furthermore, in ophthalmologic applications, OCT provides higher resolution than traditional diagnostic techniques. Optical imaging will have many advantages over existing periodontal diagnostic procedures. OCT will produce two- or three-dimensional images depicting the topology of the pocket and connective tissue attachment and can be used to assess relationships between hard and soft tissue. These visual records can be filed as a part of the patient record. Errors associated with examiner variability in pocket probing measurements will be eliminated. Furcation involvement associated with early loss of attachment can be detected, rapidly recorded and compared with subsequent readings following therapy. Fenestrations and dehiscences, often not diagnosed until surgery, can also be detected. Patient acceptance of this technique will be far greater than for most dental procedures. OCT is entirely non-invasive and can be performed repeatedly to monitor progress of therapy without any danger to the patient. Oct is painless, rapid, and can be easily incorporated into a routine dental examination. This technology will also be a powerful research tool. Oct can potentially be used to differentiate between active and inactive periodontal disease. The orientation of collagen fibers before, during and following therapy can be repeatedly assessed to evaluate the progress of periodontal treatment. Moreover these optical techniques can spectroscopically detect, quantify and monitor small concentrations of specific molecules. This potential application will permit rapid, non- invasive measurement of multiple molecules within periodontal pockets. The Specific Aims of this proposal are to: 1. Adapt existing laser equipment and technology to demonstrate that OCT can be used for periodontal diagnoses. 2. Compare diagnostic findings derived from OCT to measurements obtained with histometric methods. 3. Construct a prototype clinical periodontal OCT imaging system at a cost that is affordable for clinical dental practices.