CORE--CELL BIOLOGY

核心--细胞生物学

• 批准号: 5206174
• 负责人: LEONARD RIFAS
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5206174
• 关键词: biomedical facility; enzyme linked immunosorbent assay; human tissue; immunocytochemistry; laboratory rat; osteoblasts; osteoclasts; radioimmunoassay; tissue /cell culture; tissue /cell preparation; tissue resource /registry

The Cell Biology Core provides Program Project investigators and their supporting staff with technical support and consultation in cell culture development, preparation and experimental design, immunohistochemistry and ELISAs. The Core occupies dedicated laboratory space within the Division of Endocrinology and Bone and Mineral Diseases at the Jewish Hospital of St. Louis. The Cell Biology Core is responsible for the screening and purchasing of serum and the preparation of the various media required to prepare, culture and maintain the numerous cell lines carried in the main and media required to prepare, culture and maintain the numerous cell lines carried in the main and satellite laboratories and available for use by the investigators in each project of the Program to carry out their individual experimental protocols. Careful quality control measures have been taken to insure that media and sera are supportive of cell growth and continual attempts made to offer the investigators the "most appropriate, i.e. osteoblastic phenotype, and homogeneous cell" cultures. To continue pursuing the development of techniques and procedures which will ultimately satisfy the continued need for viable bone marrow, phenotypic rat and human osteoblasts and bone marrow stromal cells, and multiple cell lines of human and rodent origin, the basic organization of the Core has been expanded over that detailed in the previous renewal. In order to support and service the experimental protocols described in this application which are targeted to: 1) examine bone cell differentiation and communication via cell adhesion; 2) examine the regulation of osteoclast development and differentiation; 3) examine the regulation of cells differentiation by 1,25(OH)2D3; and 4) examine osteoclast-mediated bone remodeling, new technical support is proposed for the present renewal. To accomplish this goal, we plan new endeavors in the Cell Biology Core including technical support in the areas of: (1) immunohistochemical analysis of osteoblast development and osteoclast function; (2) analysis of cell culture supernatant for cytokines by ELISA; (3) providing services for the development, and preparation and/or maintenance of new cell lines, and 4) providing a centralized repository for cDNA probes. Based on current and projected usage, we expect that the Core resources will be extensively utilized and that demands for our services to grow during the next funding period.

细胞生物学核心提供程序项目研究人员和他们的 支持人员提供细胞培养方面的技术支持和咨询 开发、准备和实验设计，免疫组织化学 和ELISA。 核心占据了实验室内的专用空间， 犹太大学内分泌学、骨骼和矿物质疾病科 圣路易斯医院 细胞生物学核心负责 血清的筛选和购买以及各种 制备、培养和维持大量细胞系所需的培养基 进行主要和媒体所需的准备，培养和维护 主要实验室和卫星实验室中携带的大量细胞系 并可供该计划每个项目的研究人员使用 来执行他们各自的实验方案 细致的品质 已采取控制措施，以确保培养基和血清 支持细胞生长，并不断尝试提供 研究者“最合适的，即成骨细胞表型，和 均质细胞”培养物。 继续追求发展 技术和程序，最终将满足持续的 需要有活力的骨髓、表型大鼠和人成骨细胞， 骨髓基质细胞和多种人类和啮齿动物细胞系 起源，核心的基本组织已经扩展到 在之前的更新中详细说明。 为了支持和服务 本申请中描述的实验方案， 目的：1）检测骨细胞分化和细胞间通讯 粘附; 2）检查破骨细胞发育的调节， 3）检测细胞分化的调节， 1，25（OH）2D 3;和4）检查破骨细胞介导的骨重建，新的 建议为本次更新提供技术支持。 完成 为了实现这一目标，我们计划在细胞生物学核心领域进行新的努力，包括 技术支持领域：（1）免疫组织化学分析 成骨细胞发育和破骨细胞功能;（2）细胞分析 培养上清ELISA法检测细胞因子;（3）为 新细胞系的开发、制备和/或维持， 和4）提供cDNA探针的集中储存库。 基于 目前和预计的使用情况，我们预计核心资源将 广泛使用，并要求我们的服务在 下一个融资期。