CORE--CELL BIOLOGY LABORATORY

核心--细胞生物学实验室

• 批准号: 6338646
• 负责人: LEONARD RIFAS
• 金额: $ 24.58万
• 依托单位: BARNES-JEWISH HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6338646
• 关键词: biomedical facility; cell biology; enzyme linked immunosorbent assay; experimental designs; human tissue; immunocytochemistry; laboratory rat; osteoblasts; osteoclasts; radioimmunoassay; tissue /cell culture; tissue /cell preparation; tissue resource /registry

The Cell Biology Core provides Program Project investigators and their supporting staff with technical support and consultation in cell culture development, preparation and experimental design, immunohistochemistry, ELISAs and molecular biology techniques. The Core occupies dedicated laboratory space within the Division of Bone and Mineral Disease at Barnes-Jewish Hospital North. The Cell Biology Core is responsible for the screening and purchasing of serum and the preparation of the various media required to prepare, culture and maintain the numerous cell lines carried in the main laboratory and available for use by the investigators in each project of the Program to carry out their individual experimental protocols. Careful quality control measures have been taken to insure that media and sera are supportive of cell growth and continual attempts made to offer the investigators the "most appropriate, i.e. osteoblastic phenotype, and homogenous cell" cultures. To continue pursuing the development of techniques and procedures which will ultimately satisfy the continued need for viable bone marrow, phenotypic rat and human osteoblasts and human bone marrow stromal cells and osteoclast-like cells, and multiple cell lines of human and rodent origin, the basic organization of the Core has been expanded. In order to support and service the experimental protocols described in the application which are targeted to: 1) examine bone cell differentiation and communication via cell adhesion; 2) examine the control of osteoclast activation and differentiation; 3) examine cell matrix response to mechanical loading; and 4) examine osteoclast-mediated bone remodeling, new technical support is proposed for the present renewal. To accomplish this goal, we plan new endeavors in the Cell Biology Core including technical support in the areas of: (1) immunohistochemical analysis of osteoblast development, expression of specific membrane proteins and osteoclast function; (2) analysis of cell culture supernatants for cytokines by ELISA; (3) providing services for the development, preparation and/or maintenance of new cell lines, and 4) providing a centralized area for molecular biology procedure. Based on current and projected usage, we expect that the Core resources will be extensive utilized nd that demands for our services to grow.

细胞生物学核心为计划项目调查员及其 为员工提供细胞培养方面的技术支持和咨询 发展，准备和实验设计，免疫组织化学， ELISA和分子生物学技术。核心占据着专门的 骨和矿物病科内的实验室空间 巴恩斯-犹太医院北部。细胞生物学核心负责 血清的筛选、购买及各种介质的制备 需要准备、培养和维护所携带的众多细胞系 在主实验室，并可供每个实验室的研究人员使用 项目开展个人实验的方案 协议。已采取谨慎的质量控制措施，以确保 培养液和血清支持细胞生长和持续的尝试 为研究人员提供“最合适的，即成骨细胞 表型和同源细胞培养。继续追求 开发技术和程序，以最终满足 继续需要存活的骨髓、表型大鼠和人类 成骨细胞、人骨髓基质细胞和破骨细胞样细胞， 和多个人类和啮齿动物起源的细胞系的基本组织 核心的一部分已经扩展。为了支持和服务于 本申请中描述的实验协议的目标是： 1)通过细胞黏附检测骨细胞的分化和通讯； 2)研究破骨细胞激活和分化的调控；3) 检查细胞基质对机械载荷的响应；以及4)检查 破骨细胞介导的骨重建，提出了新的技术支持 目前的更新。为了实现这一目标，我们计划在 细胞生物学核心包括以下方面的技术支持：(1) 成骨细胞发育的免疫组织化学分析，表达 特异膜蛋白与破骨细胞功能；(2)细胞分析 细胞因子培养上清的酶联免疫吸附试验；(3)为 开发、准备和/或维护新的细胞系，以及4) 为分子生物学程序提供集中区。基于 当前和预计的使用情况，我们预计核心资源将是 广泛使用，我们的服务需求也在不断增长。