MAMMALIAN GENOME MAPPING OF TISSUE SPECIFIC CDNAS

描述(改编自《研究人员摘要》)：人类基因组 项目正在进入测序密集阶段。然而，它是 这一建议的前提是基于高分辨率基因和EST的图谱 建筑业仍然可以在1)提供额外的 这些努力的基础设施，以及2)促进利用 在广泛的遗传学研究中出现的基因组资源 疾病。计算机算法将用于识别小鼠EST/基因 这是绘制的人类EST/基因的同源同源物。为了进一步开发一种 研究人员将绘制高密度基因丰富的小鼠基因组图谱 600个这样的小鼠EST/基因使用它们特征良好的种间 回交(包含1200个标记)。选定要映射的鼠标Est 将基于同源人类的人类染色体位置 单胞菌。尽管同源关系已在 低分辨率保守基因组片段的边界以及 部门内重组事件在很大程度上仍然是模棱两可的。海流 提案将允许这些鼠标/人的更高分辨率清晰度 通过EST和基因的选择性定位建立的同源关系 单基因密集小鼠图谱。微卫星标记在中国的整合 高密度遗传图谱也将是这些努力的一个组成部分。 将选择大约500个微卫星标记来提供 这些地图的整合。这些研究将促进重叠群 小鼠的基因构建以及小鼠和小鼠的基因搜寻研究 人类。该提案将为未来提供一个额外的框架 生物学研究以及必要的工具来验证和推广 小鼠基因组的确定特征和重叠群的完成 建筑。提供对人/鼠标的访问的在线数据库 同源性(http：//www3.ncbi.nlm.nih.gov/homology/)将保持不变 作为此项目的一部分进行了更新。该数据库将提供一个简单的 人和小鼠编码序列以及非基因STS之间的接口 记号笔。该数据库将与该组织的在线基因图谱相结合。 人类基因组，并将添加注释，以包括未映射的小鼠同源基因 人类单基因及其推测的染色体位置。

DESCRIPTION (Adapted from the Investigator's Abstract): The human genome project is entering a sequencing intensive stage. However, it is the premise of this proposal that high resolution gene and EST based map construction still has a role to play in 1) providing additional infrastructure for these efforts and 2) to facilitate the utilization of the emerging genome resources in a wide variety of investigations into genetic disease. Computational algorithms will be used to identify mouse ESTs/genes that are the orthologues of mapped human ESTs/genes. To further develop a high density gene rich map of the mouse genome the investigators will map 600 of these mouse ESTs/genes using their well-characterized interspecific backcross (containing 1200 markers). The mouse ESTs selected for mapping will be based on the human chromosomal location of the orthologous human unigenes. Although homology relationships have been rapidly identified at low resolution the borders of conserved genomic segments as well as intra-segment rearrangement events remain largely ambiguous. The current proposal will allow a higher resolution definition of these mouse/human homology relationships by this selective mapping of ESTs and genes in a single gene dense mouse map. The integration of microsatellite markers in the high-density genetic map will also be a component of these efforts. Approximately 500 microsatellite markers will be chosen to provide an integration of these maps. These studies will facilitate contig construction in the mouse as well as gene hunting studies in both mouse and human. The proposal will provide an additional framework for future biological studies as well as the tools necessary to verify and extend the definitive characterization of the mouse genome and completion of contig construction. An on-line database that provides access to the human/mouse homologies (http:/www3.ncbi.nlm.nih.gov/Homology/) will be maintained and updated as part of this project. This database will provide a simple interface between human and mouse coding sequences as well as nongenic STS markers. The database will be integrated with the online Gene Map of the Human Genome and will be annotated to include unmapped mouse orthologues of human unigenes and their putative chromosomal location.