Validation of LC-MS/MS analyses of animal tissue and feed matrices for toxicants

验证动物组织和饲料基质中有毒物质的 LC-MS/MS 分析

• 批准号: 8908915
• 负责人: Cynthia Lynn Gaskill
• 金额: $ 9.9万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2018-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8908915
• 关键词: Validation; LC; MS; analyses; animal

2013 Gaskill Vet-LIRN Project Summary/Abstract The Veterinary Laboratory Investigation and Response Network (Vet-LIRN) was developed by the FDA to increase the safety of human and animal health by investigating potential adverse events involving animal feed or animal drug contamination or adulteration. The testing of diagnostic specimens adds insight into investigations not routinely obtained from traditional food testing laboratories. Such investigations require detection methods that are validated for organs and diagnostic samples such as urine, feces, and blood, which are not typical food matrices. The objective of the proposed work is to expand the number of multi-laboratory validated analytical methods that can be used by the FDA Vet-LIRN cooperative laboratories during outbreaks or events of animal feed or drug contaminations. The proposed project will also strengthen the collaborations and integration of the network laboratories to encourage seamless interactions during actual emergency related testing. The specific aim of this proposal is to perform multi-laboratory validations of liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods for detection and quantitation of anticoagulant rodenticides in animal tissues, fluids and feeds, and mycotoxins in animal tissues and feeds. Analytes and matrices for these methods will be expanded in the last year of the project as needed to address specific Vet-LIRN needs. Another major aim of this proposal is to provide collaborative support for other funded Vet-LIRN multi- laboratory method validation projects. We have agreed to collaborate and provide support of seven other Vet- LIRN network laboratory toxicology sections. This proposal will help ensure that the University of Kentucky Veterinary Diagnostic Laboratory has the necessary instrumentation and technical staff support to adequately handle the collaborative analyses. The expected outcomes are to complete the multi-laboratory validation of the methods listed above and disseminate the methods to Vet-LIRN collaborating laboratories and to the FDA Vet-LIRN. The impact of the proposed work will be to improve human and animal health by increasing the number of validated testing methods available for collaborating Vet-LIRN laboratories to use during outbreaks or events of animal feed or drug contamination or adulterations.

2013 Gaskill Vet-LIRN项目摘要/摘要 兽医实验室调查和响应网络（Vet-LIRN）由FDA开发， 通过调查涉及动物饲料的潜在不良事件，提高人类和动物健康的安全性 或动物药物污染或掺假。诊断样本的测试增加了对以下方面的了解： 传统的食品检测实验室没有进行常规的调查。这种调查需要 对器官和诊断样本（如尿液、粪便和血液）进行验证的检测方法， 不是典型的食物基质。 拟议工作的目标是扩大多实验室验证的分析方法的数量 FDA Vet-LIRN合作实验室在动物饲料爆发或事件期间可以使用的， 毒品污染。拟议项目还将加强 网络实验室，以鼓励在实际紧急情况相关的测试过程中进行无缝互动。 本提案的具体目的是进行液相色谱-串联的多实验室验证 动物体内抗凝血杀鼠剂的质谱检测方法 组织、液体和饲料，以及动物组织和饲料中的霉菌毒素。分析物和基质 在项目的最后一年，将根据需要扩展方法，以满足特定的Vet-LIRN需求。 该提案的另一个主要目的是为其他受资助的Vet-LIRN多学科提供协作支持， 实验室方法验证项目。我们已经同意合作，并提供其他七个兽医的支持- LIRN网络实验室毒理学部分。这项提案将有助于确保肯塔基州大学 兽医诊断实验室拥有必要的仪器和技术人员支持， 处理协作分析。 预期成果是完成上述方法的多实验室验证， 向Vet-LIRN合作实验室和FDA Vet-LIRN传播方法。的影响 拟议的工作将是通过增加有效测试的数量来改善人类和动物的健康 在动物饲料爆发或事件期间，可供Vet-LIRN合作实验室使用的方法，或 药物污染或掺假。