LINKAGE MAPPING HUMAN TELOMERES AND CENTROMERES

描述：(改编自《调查者摘要》)：长期 这个项目的目标是充分描述人类的目的 染色体。这些调查的结果之一将是建造 完全集成的区域遗传和物理地图，最终 DNA序列中的分辨率。未来三年的目标是 开发初级资源全面完善端粒图谱 (克隆、遗传标记、STSS)和构建基线综合地图。 选定的端粒区域将被密集绘制，研究人员 将测试从几个地区进行基因组DNA测序的可行性 与终端T2AU3相邻的位置重复。此外，他们还提议 维护一个全面的电子端粒资源，TeIDB，它将 促进端粒数据在 科学界。下一步要实现的具体目标 三年时间：1)继续对选定的人类端粒进行基因作图 开发高信息量的端粒微卫星标记 尚未报告此类标记的区域。新的 微卫星标记将被纳入多点和2D 交叉的减数分裂端粒区域图谱。2)描述基因组 结构或选定的人类端粒区域。3)利用所有可用的资源 物理和基因测绘数据，以产生统一的区域物理和遗传图谱 基因端粒图谱。4)确定端粒的候选克隆 哪些克隆人还没有被识别。5)维护端粒 数据库资源TeIDB，目前在互联网上可用 (http://www.genlink.wustl.edu/teldb/).)

Description: (adapted from the Investigator's Abstract): The long-term objective of this project is to fully characterize the ends of human chromosomes. One outcome of these investigations will be construction of fully integrated regional genetic and physical maps with ultimate resolution in the DNA sequence. The goals for the next three years are to generally improve the telomere maps by developing primary resources (clones, genetic markers, STSs) and constructing baseline integrated maps. Selected telomere regions will be intensively mapped and the investigators will test the feasibility of sequencing genomic DNA from several regions adjacent to the terminal T2AU3 repeats. In addition, they propose to maintain a comprehensive electronic telomere resource, TeIDB, that will facilitate communication and sharing of telomere data within the scientific community. The specific aims to be achieved during the next three years are to: 1) Continue genetic mapping of selected human telomere regions and develop highly informative microsatellite markers for telomere regions for which such markers have not yet been reported. The new microsatellite markers will be incorporated into multipoint and 2D crossover meiotic telomere region maps. 2) Characterize the genomic structure or selected human telomere regions. 3) Utilize all available physical and genetic mapping data to produce unified regional physical and genetic telomere maps. 4) Identify candidate clones for telomeres for which clones have not yet been identified. 5) Maintain the telomere database resource, TeIDB, which is currently available over the intemet (http://www.genlink.wustl.edu/teldb/).