FRAMEWORK LINKAGE MAPS FOR CHROMOSOMES 2 6 7 8 12 & 14

染色体 2 6 7 8 12 的框架连锁图

• 批准号: 3333656
• 负责人: HELEN R DONIS-KELLER
• 金额: $ 101.62万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-04-01 至 1994-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3333656
• 关键词: chromosomes; computer assisted sequence analysis; cytogenetics; endonuclease; gender difference; genetic library; genetic manipulation; genetic markers; genetic polymorphism; genetic techniques; genotype; human genetic material tag; human population genetics; human tissue; image processing; in situ hybridization; linkage mapping; method development; nucleic acid probes; nucleic acid sequence; oligonucleotides; polymerase chain reaction; restriction fragment length polymorphism; southern blotting

We propose to construct framework genetic linkage maps for human chromosomes 2, 6, 7, 8, 12, and 14. The maps will consist of markers for DNA sequence polymorphism with minimum heterozygosities of 70% and marker spacing, in general, of no more than 1 0% recombination. Slightly higher recombination intervals will be allowed provided marker informativeness is considerably higher than 70%. Initial maps (male, female, and sex average) will be constructed from CEPH reference pedigree genotypes using the linkage analysis program package CRI-MAP and these will serve as the basis for determining regions requiring probe development. CA repeat polymorphisms will be the main type of new markers to be identified and characterized. Semiautomated image analysis programs will be developed as an aid to genotype analysis for CA polymorphisms. Initial sets of candidate index markers from each chromosome are proposed for study. A novel approach to test the feasibility of bi-allelic markers and automated genotyping assays is proposed for chromosome 14 mapping in conjunction with more traditional approaches.

我们建议构建人类框架遗传连锁图谱 染色体 2、6、7、8、12 和 14。图谱将包含以下标记： 最小杂合度为 70% 的 DNA 序列多态性和标记 一般来说，重组间距不超过 1 0%。 稍高一点 如果标记信息丰富，则允许重组间隔 远高于70%。 初始地图（男性、女性和性别） 平均值）将使用 CEPH 参考谱系基因型构建 连锁分析程序包 CRI-MAP，这些将作为 确定需要探针开发的区域的基础。 CA重复 多态性将是待鉴定的新标记的主要类型 特点。半自动图像分析程序将开发为 有助于 CA 多态性的基因型分析。 初始组 建议研究每条染色体的候选索引标记。 一个 测试双等位标记可行性的新方法 建议使用自动基因分型测定法进行 14 号染色体定位 与更传统的方法结合。