GENETIC ANALYSES OF EBV'S IMMORTALIZING GENES

描述（改编自申请人的摘要）： 通过以下途径促进人类B淋巴细胞永生化的病毒基因 由于缺乏可靠的手段， 用于在不存在污染性野生型病毒的情况下测试突变病毒。 博士萨格登与他的长期同事，博士。 Hammerschellen已经开发出EBV的衍生物，称为迷你EBV，其可以 在E.大肠杆菌，包装在人细胞系中 含有EBV辅助病毒，并且能够单独永生化 B淋巴细胞 定义的突变可以在其表达中引入到迷你EBV中。 E. coli宿主。 萨格登博士和哈默施泰特博士建议测量 在选定的EBV永生化基因中确定的突变对效率的影响 永生的可能性 这些基因也将被分析，以确定是否 它们是启动和/或维持 永生 最后，含有最少量的 将构建永生化所需的遗传信息。

DESCRIPTION (Adapted from Applicant's Abstract): Genetic analysis of the viral genes that contribute to the immortalization of human B-lymphocytes by Epstein-Barr virus (EBV) has been hampered by the lack of a reliable means for testing mutant viruses in the absence of contaminating wild-type virus. Dr. Sugden, in collaboration with his longstanding colleague, Dr. Hammerschmidt have developed derivatives of EBV, termed mini-EBVs, that can be propagated as plasmids in E. coli, packaged in a human cell line containing an EBV helper virus, and are capable alone of immortalizing B-lymphocytes. Defined mutations can be introduced into mini-EBVs in their E. coli host. Drs.Sugden and Hammerschmidt propose to measure the effects of defined mutations in selected EBV immortalizing genes on the efficiency of immortalization. These genes will also be analyzed to determine whether they are required for the initiation and/or the maintenance of immortalization. Finally, mini EBVs containing the minimal amount of genetic information required for immortalization will be constructed.