Development of a highly multiplexed optical detection system for analysis of complex, PCR-based DNA tests.

开发高度多重光学检测系统，用于分析基于 PCR 的复杂 DNA 测试。

• 批准号: 710092
• 金额: $ 12.59万
• 依托单位: B G RESEARCH LIMITED
• 依托单位国家: 英国
• 项目类别: GRD Proof of Concept
• 财政年份: 2011
• 资助国家: 英国
• 起止时间: 2011 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=710092
• 关键词: Development; highly; multiplexed; optical; detection

Recent increases in genomic information has led to an exponential increase in the numbers ofgenetic diseases characterised. This and a greater understanding of the infection process,generates the need to detect larger numbers of targets such as pathogens and disease causinggenetic variants as rapidly as possible and in a single test.One such approach commonly in use is real-time PCR and yet the application of this formultiplex testing has been limited by inherent flaws in existing detection systems, cost of thetechnology, and the time taken to generate results.The design and manufacture of a rapid and low cost optical system capable of concurrentlyanalysing a large number of targets is the goal of this study. Such an approach would reducethe cost of such testing, by allowing more targets to be screened in a single test and wouldreduce the cost for uptake of the technology, as well as reducing the time to result to the pointit could be performed "while you wait", for example at a doctors surgery.Similarly this would allow novel PCR based techniques, previously impossible byconventional real-time PCR. Examples could include forensics, by virtue of being able toanalyse more targets in a single run. This could also be applied to complex medicaldiagnostics such as mixed infections.The system would inherently also be many times faster than existing approaches, since itcould detect and deconvolute all visible wavelengths of light in a single reading as opposed tohaving to switch between a series of filters which greatly increases the analysis time oncurrent instrumentation as well as reducing the number of analytes that can concurrently betested for.In essence this proof of concept will use expertise in the field of satellite imaging andmathematical modelling in conjunction with our Ultra-Rapid thermal cycling IP to solve theproblem of multiplex detection of labelled PCR targets and hence allow unique targeting of agrowing market niche.

最近基因组信息的增加导致了特征性遗传疾病数量的指数增加。这一点以及对感染过程的更深入了解，产生了在一次测试中尽可能快速地检测大量目标（如病原体和致病基因变异）的需求。一种常用的方法是实时PCR，但这种方法的应用受到现有检测系统固有缺陷、技术成本、以及产生结果所需的时间。设计和制造能够同时分析大量目标的快速、低成本光学系统是本研究的目标。这种方法可以通过允许在一次测试中筛选更多的目标来降低这种测试的成本，并且可以降低采用该技术的成本，同时可以将得出结果的时间缩短到可以在“等待时”进行的程度，例如在医生的手术中。同样地，这将允许基于PCR的新技术，以前传统的实时PCR是不可能的。这方面的例子可以包括法医学，因为它能够在一次运行中分析更多的目标。这也可以应用于复杂的医学诊断，如混合感染。该系统本身也比现有方法快许多倍，因为它可以在单个阅读中检测和去卷积所有可见波长的光，而不是必须在一系列滤光片之间切换，这大大增加了当前仪器的分析时间，并减少了可以同时测试的分析物的数量。这一概念验证将利用卫星成像和数学建模领域的专业知识，结合我们的超快速热循环IP，解决标记PCR靶标的多重检测问题，从而实现对不断增长的市场利基的独特定位。