MLCT EXCITED STATES AS LUMINESCENT BIOPHYSICAL PROBES

MLCT 激发态作为发光生物物理探针

• 批准号: 2021432
• 负责人: FELIX N CASTELLANO
• 金额: $ 2.43万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-10-03 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2021432
• 关键词: biophysics; charge transfer complex; chemical synthesis; copper; fluorescence spectrometry; fluorescent dye /probe; heavy metals; ligands; luminescence; nucleic acid probes; osmium; ruthenium

The primary goal of this research is to develop and implement novel luminescent metal-to-ligand charge transfer (MLCT) compounds as extrinsic biophysical probes. The luminescence properties of these compounds can be dramatically altered and well controlled with variation of the lignads and/or metal center. This coupled with the favorable characteristics of MLCT compounds (visible absorbance bands, high quantum yields, long lifetimes, and polarized emission) makes them ideal candidates for use in biophysical spectroscopy. Thee compounds provide a unique opportunity to explore biophysical phenomena on time scales that are largely unexplored. Initial studies will focus on the factors which control the emission anisotropy of MLCT compounds. This will be followed by the synthesis of MLCT compounds which have conjugatable ligands for attachment to biomolecules. Each compound will be designed with specific intrinsic properties such as long wavelength luminescence or extremely long lifetime. All dynamical measurements will be performed in a complimentary manner, in both the time and frequency domain.

这项研究的主要目标是开发和实施小说 发光金属-配体电荷转移(MLCT)化合物AS 外在生物物理探头。这些化合物的发光特性 化合物可以被戏剧性地改变并得到很好的控制 木头和/或金属中心的变化。这一点再加上 MLCT化合物的良好特性(可见光吸光度 频带、高量子产额、长寿命和极化发射) 这使它们成为生物物理光谱学的理想候选者。你 化合物为探索生物物理提供了一个独特的机会 时间尺度上的现象在很大程度上是未被探索的。初步研究 将集中在控制发射各向异性的因素上 MLCT化合物。随后将合成MLCT 具有可结合配体的化合物 生物分子。每个院落都将被设计成 特定的本征特性，如长波长发光或 极其漫长的一生。所有动态测量都将是 在时间和频率上都以赞扬的方式表演 域。