MLCT COMPOUNDS AS LUMINESCENT BIOPHYSICAL PROBES

在过去的几年里，我们专注于发展 金属-配体电荷转移(MLCT)化合物作为非本征化合物 用于生物化学和生物物理的发光探头。这些是基于 在d6上，过渡金属中心(Ru(II)、Os(II)和Re(I))被螯合到 是二亚胺的配体，如2，2‘-联吡啶(BPY)和其他 单齿或双齿配体。我们已经采取了几种方法 为了开发这类化合物作为发光材料用于 生物分子。我们目前已经分离出三种胺类反应物和两种 已共价连接到三个基团的巯基反应配体 不同的金属中心，并随后与几个类别反应 生物分子。这些探头正被用于开发新的临床 化学分析，并正在分发到其他实验室。

Over the past years we focused our effort on development of metal-to-ligand charge transfer (MLCT) compounds as extrinsic luminescence probes for biochemistry and biophysics. These are based on d6 transition metal centers (Ru(II), Os(II), and Re(I)) chelated to ligands which are diimines, such a 2,2'-bipyridine (bpy), and other mono-or bidentate ligands. There are several approaches we have taken in order to develope this class of compounds as luminescence for biomolecules. We have presently isolated three amine-reactive and two sulfhydryl-reactive ligands that have been covalently linked to three different metal centers, and subsequently reacted with several classes of biomolecules. These probes are being used to develop new clinical chemistry assays, and are being distributed to other laboratories.