MLCT COMPOUNDS AS LUMINESCENT BIOPHYSICAL PROBES

过去几年，我们集中力量发展 金属-配体电荷转移（MLCT）化合物作为外源性 用于生物化学和生物物理学的发光探针。 这些都是基于 在d6过渡金属中心（Ru（II）、Os（II）和Re（I））上螯合， 为二亚胺的配体，例如2，2'-联吡啶（bpy），以及其它配体， 单齿或双齿配体。 我们采取了几种方法 为了开发这类化合物作为发光材料， 生物分子 我们目前已经分离出三种胺反应性和两种 巯基反应性配体已经共价连接到三个 不同的金属中心，并随后与几类 生物分子。 这些探针被用于开发新的临床 化学分析，并正在分发给其他实验室。

Over the past years we focused our effort on development of metal-to-ligand charge transfer (MLCT) compounds as extrinsic luminescence probes for biochemistry and biophysics. These are based on d6 transition metal centers (Ru(II), Os(II), and Re(I)) chelated to ligands which are diimines, such a 2,2'-bipyridine (bpy), and other mono-or bidentate ligands. There are several approaches we have taken in order to develope this class of compounds as luminescence for biomolecules. We have presently isolated three amine-reactive and two sulfhydryl-reactive ligands that have been covalently linked to three different metal centers, and subsequently reacted with several classes of biomolecules. These probes are being used to develop new clinical chemistry assays, and are being distributed to other laboratories.