MLCT EXCITED STATES AS LUMINESCENT BIOPHYSICAL PROBES

MLCT 激发态作为发光生物物理探针

• 批准号: 2701474
• 负责人: FELIX N CASTELLANO
• 金额: $ 1.14万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2701474
• 关键词: biophysics; charge transfer complex; chemical synthesis; copper; fluorescence spectrometry; fluorescent dye /probe; heavy metals; ligands; luminescence; nucleic acid probes; osmium; ruthenium

The primary goal of this research is to develop and implement novel luminescent metal-to-ligand charge transfer (MLCT) compounds as extrinsic biophysical probes. The luminescence properties of these compounds can be dramatically altered and well controlled with variation of the lignads and/or metal center. This coupled with the favorable characteristics of MLCT compounds (visible absorbance bands, high quantum yields, long lifetimes, and polarized emission) makes them ideal candidates for use in biophysical spectroscopy. Thee compounds provide a unique opportunity to explore biophysical phenomena on time scales that are largely unexplored. Initial studies will focus on the factors which control the emission anisotropy of MLCT compounds. This will be followed by the synthesis of MLCT compounds which have conjugatable ligands for attachment to biomolecules. Each compound will be designed with specific intrinsic properties such as long wavelength luminescence or extremely long lifetime. All dynamical measurements will be performed in a complimentary manner, in both the time and frequency domain.

本研究的主要目标是开发和实施新的 发光金属-配体电荷转移（MLCT）化合物， 外源性生物物理探针。 它们的发光特性 化合物可以显着改变和良好的控制， 配体和/或金属中心的变化。 这与 MLCT化合物的有利特性（可见吸光度 带，高量子产率，长寿命和偏振发射） 使它们成为生物物理光谱学的理想候选者。 你 化合物提供了一个独特的机会，探索生物物理 在时间尺度上的现象，在很大程度上是未探索的。 初步研究 将集中在控制发射各向异性的因素， MLCT化合物。 随后将进行MLCT的合成 具有可缀合配体的化合物 生物分子 每个建筑群的设计都将 例如长波长发光或 极长的寿命。 所有的动态测量将是 在时间和频率上以互补的方式执行 域

项目成果

A water-soluble luminescence oxygen sensor.

水溶性发光氧传感器。

• DOI: 10.1562/0031-8655(1998)067
• 发表时间: 1998
• 期刊: Photochemistry and photobiology
• 影响因子: 3.3
• 作者: Castellano,FN;Lakowicz,JR
• 通讯作者: Lakowicz,JR