MOLECULAR AND GENETIC ANALYSIS OF DROSOPHILA LIM-1

果蝇 LIM-1 的分子和遗传分析

• 批准号: 2459272
• 负责人: Brenda J Lilly
• 金额: $ 2.01万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2459272
• 关键词: Drosophilidae; biomarker; developmental genetics; developmental neurobiology; gene mutation; genetic mapping; invertebrate cuticle; larva; polymerase chain reaction; transcription factor

The goal of this fellowship is to ascertain the role of the Lim- l gene product in Drosophila head and nervous system development. The objectives in doing so are, to define the specific role Lim-l has in these processes, advance the current knowledge of head and nervous system development, and identify parallels to the vertebrate Lim- l gene. The specific aims are designed to concisely address each of these issues. Specific aim #1 will examine the temporal and spatial relationship of Drosophila Lim-l with respect to relevant developmental regulators. By immunostaining for Lim-l in wild-type and mutant Drosophila embryos, the genetic and molecular position of Lim-l can be determined and placed in an appropriate hierarchy. Specific aim #2 will entail the molecular and genetic mapping of the Lim- l locus, and the generation of a loss-of-function mutations by genetic and PCR screens. Specific aim #3 will address the consequent phenotype of the Lim-l mutations. The analysis will focus on defining the molecular and morphological phenotypes observed in head formation and nervous system development. This will be done by staining for pertinent markers and analyzing cuticle structures of the Lim-l mutant embryos. These studies will reveal new and important information about how the LIM class transcription factor, Lim-l functions in Drosophila development, and will provide a clearer understanding of the similarities that exist between Drosophila and vertebrates.

这项研究的目标是确定LIM-L基因的作用 产品在果蝇头部和神经系统发育。目标 在这样做的过程中，为了定义林-L在这些过程中所扮演的具体角色， 增进当前有关头部和神经系统发育的知识，以及 鉴定与脊椎动物LIM-L基因的同源性。具体目标是 旨在简明扼要地解决这些问题。具体目标#1将 果蝇Lim-L的时空关系研究 尊重相关的发育调节器。LIM-L的免疫组织化学染色 在野生型和突变型果蝇胚胎中，遗传和分子 可以确定林-L的位置，并将其置于适当的等级中。 具体目标2将涉及LIM的分子和遗传图谱- L基因座，并产生一种功能缺失突变，由基因和 聚合酶链式反应筛查。具体目标#3将解决由此产生的 林-L突变。分析将集中在定义分子和 头部结构和神经系统的形态表型观察 发展。这将通过染色相关的标记和 分析Lim-L突变胚胎的角质层结构。这些研究 将揭示有关LIM类如何 转录因子LIM-L在果蝇发育中发挥作用，并将 更清楚地了解以下方面存在的相似性 果蝇和脊椎动物。