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3D quantitative tomographic microscopy of low-contrast structures

低对比度结构的 3D 定量断层显微术

基本信息

批准号：
710152
负责人：
金额：
$ 12.74万
依托单位：
PHASE FOCUS LIMITED
依托单位国家：
英国
项目类别：
GRD Proof of Concept
财政年份：
2012
资助国家：
英国
起止时间：
2012 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=710152
关键词：
3D quantitative tomographic microscopy low

项目摘要

The Phase Focus Virtual Lens® (otherwise known as ptychography) is a new microscopymethod that eliminates the need for hardware focusing components, thereby removing thestraitjacket imposed by the costs, limitations and imperfections of conventional lenses.Instead of lenses, the Virtual Lens image reconstruction process is performed by asophisticated computer programme (algorithm.) Because it provides quantitative (numerical)information, and because it can extract the high information content often present intransparent specimens, the Virtual Lens has advantages in applications as diverse as live cellimaging and ophthalmic (contact) lens manufacture.The existing Virtual Lens algorithm relies on a two-dimensional mathematical approximationof the specimen. If the specimen’s structure is physically three-dimensional, or if it is thick,poor image quality can result. The purpose of this work, therefore, is to produce fulltomographic quantitative images of low-contrast specimens of complex structures, using anew three-dimensional Virtual Lens algorithm.If successful, it is expected that this new method will be able to generate high quality andaccurate information from such specimens without the use of stains, and from greater depthswithin the specimens than can be observed using conventional confocal microscopes.In addition, the technique is expected to be able to provide a new way to measure accurately,for manufacturing quality assurance purposes, the full three dimensional shape of lenses thatare used in CD and DVD players, mobile phones, microscopes, telescopes and other products.It is anticipated that this work will therefore create new and improved products and processesfor the benefit of UK industry and society at large.

Phase Focus Virtual Lens®（也称为 ptychography）是一种新的显微方法，无需硬件聚焦组件，从而消除了传统镜头的成本、限制和缺陷所带来的束缚。虚拟镜头图像重建过程不是通过镜头，而是通过复杂的计算机程序（算法）来执行。因为它提供了定量的图像（数字）信息，并且由于它可以提取通常存在于透明样本中的高信息含量，虚拟镜头在活细胞成像和眼科（接触）镜片制造等多种应用中具有优势。现有的虚拟镜头算法依赖于样本的二维数学近似。如果样本的物理结构是三维的，或者很厚，则可能会导致图像质量较差。因此，这项工作的目的是使用新的三维虚拟镜头算法生成复杂结构的低对比度标本的全断层定量图像。如果成功，预计这种新方法将能够在不使用染色的情况下从此类标本中生成高质量和准确的信息，并且从标本内比使用传统共焦显微镜观察到的深度更大。出于制造质量保证的目的，该技术预计能够提供一种新的方法来精确测量用于 CD 和 DVD 播放器、手机、显微镜、望远镜和其他产品的透镜的完整三维形状。因此，预计这项工作将创造新的和改进的产品和工艺，造福英国工业和整个社会。