THYROID SODIUM I/ SYMPORTER

描述(改编自申请者的摘要)：远距离 这个项目的目标是详细的分子表征 Na+/I-转运蛋白(NIS)，一种催化活性的关键转运蛋白 将碘转移到甲状腺中。在之前的资助中 在此期间，这一项目已经取得了相当大的进展。A基因 编码NIS的基因已被克隆，并在卵母细胞和 因为细胞。已经提出了国家情报机构的结构模型，第一个 已经产生了抗NIS抗体。此外，电生理学 NIS的分析已经在卵母细胞中进行，而 对NIS功能中的天冬氨酸#16残基进行了研究。这个 本申请的具体目的是：1)继续 功能重要氨基酸残基及其结构域的鉴定 NIS并研究其在质膜中的拓扑结构，2)阐明 NIS的生物发生和调控机制，以及3)探索可能的 不同组织的碘转运体之间的同源性，包括 唾液腺中存在的甲状腺外碘蓄积系统 和胃粘膜。

DESCRIPTION (Adapted from the applicant's abstract): The long-range objective of this project is the detailed molecular characterization of the Na+/I-symporter (NIS), a key transport protein that catalyzes the active translocation of iodide into the thyroid gland. In the previous funding periods, considerable progress has been made on this project. A cDNA encoding the NIS has been cloned, and functionally expressed in oocytes and COS cells. A structural model for the NIS has been proposed, and the first anti-NIS antibodies have been generated. In addition, electrophysiological analysis of NIS has been carried out in oocytes, and the importance of the aspartic acid #16 residue in NIS function has been investigated. The specific aims of the present application are: 1) to continue the identification of functionally important amino acid residues and domains of NIS and investigate its topology in the plasma membrane, 2) to elucidate the biogenesis and regulatory mechanisms of NIS, and 3) to explore possible homologies between iodine transporters from different tissues, including the extra-thyroidal iodine accumulation systems present in the salivary gland and gastric mucosa.