ASTROGLIAL REACTION TO ISCHEMIC BRAIN INJURY
ASTROGLIAL REACTION TO ISCHEMIC BRAIN INJURY
批准号:
2037109
负责人:
Richard P Kraig
金额:
$22.02万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1983
资助国家:
美国
项目状态:
已结题
起止时间:
1983-04-01 至 1998-11-30
关键词:
acid base balance astrocytes bicarbonates biomarker bromodeoxyuridine cerebral ischemia /hypoxia disease /disorder model dyes electrophysiology enzyme linked immunosorbent assay fluorescence microscopy glial fibrillary acidic protein hippocampus hypercapnia immunocytochemistry induced hypothermia laboratory rat membrane potentials microelectrodes monoclonal antibody neural degeneration neurophysiology potassium pyramidal cells spreading cortical depression stainings statistics /biometry western blottings
中文摘要
星形胶质细胞是高度相互作用和反应性的脑细胞
英文摘要
Astrocytes are highly interactive and reactive brain cells that are
essential for brain function. Accordingly, damage to brain will evolve
directly from injury to astrocytes or these cells will modify their
behavior in response to injury of adjacent cells. Examination of how
astrocytes respond to injury from brain ischemia should lead to an improved
understanding of the cellular pathogenesis of this malady. This philosophy
guides the long term goal of this project: To understand how astrocytes
respond to ischemic brain injury and how acid-base fluctuations participate
in their response.
Astrocytes either degenerate from severe ischemia (i.e. infarction) or they
are transformed into reactive species by less lethal reduction in flow.
Little physiologic information exists that characterizes these two cell
changes or alludes to what causes the alterations. Since H+i and K+i
modulate vital activities in eukaryotic cells, are interrelated in
astrocytes, and are two principal ionic species regulated by these cells,
the interrelation of changes in astrocytic H+i and K+i to ischemic injury
will be examined in detail here. Ion-selective microelectrodes for pH, K+,
as well as a newly developed triple barrel microelectrode array sensitive
to pH & CO32, will be used to correlate the patterns of astrocytic acid-
base and K+ change from ischemia that are associated with structural
markers for these cells (i.e measurements of the cell body size, degree of
DNA replication, intensity and distribution of GFAP staining as well as
change in regional brain GFAP content). Degeneration of astrocytes will be
studied in vivo using a rodent model of focal infarction since this type of
ischemic brain injury is clinically most prevalent. Astrocytes become
severely acidotic and lose their processes during infarction from global
ischemia, perhaps because of a critical reduction in [HCO3-]i. How
astrocytic pHi, [HCO3-]I and morphology change with focal infarction is
unknown and will be determined. Astrocytic changes in H+i and K+i
associated with reactive astrocytosis will be studied in vivo using global
ischemia and correlated to the above structural markers of astrocytosis.
Furthermore, the effect of hypothermia, which retards neuronal destruction
from global ischemia, on ionic and structural variables associated with
reactive astrocytosis will be examined since such destruction is thought to
be necessary for astrocytic proliferation. Additionally, since astroglial
pHi rises at the onset of reactive astrocytosis, the effect of hypercarbia,
which acidifies these cells will be studied. Finally, the in vitro brain
slice preparation will be used to determine plasma membrane-based
mechanisms which interrelate changes in astrocytic pHi and K+i.
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会议论文
Exosome RNA-Therapeutics to Promote CNS Myelination
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批准号:8811811
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项目类别:
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资助金额:$7.9万
-
财政年份:2013
-
负责人:Richard P Kraig
-
依托单位:
Exosome RNA-Therapeutics to Promote CNS Myelination
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批准号:9128775
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项目类别:
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资助金额:$32.35万
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财政年份:2013
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负责人:Richard P Kraig
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依托单位:
Exosome RNA-Therapeutics to Promote CNS Myelination
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批准号:9060634
-
项目类别:
-
资助金额:$7.9万
-
财政年份:2013
-
负责人:Richard P Kraig
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依托单位:
Exosome RNA-Therapeutics to Promote CNS Myelination
-
批准号:8582007
-
项目类别:
-
资助金额:$24.85万
-
财政年份:2013
-
负责人:Richard P Kraig
-
依托单位:
Exosome RNA-Therapeutics to Promote CNS Myelination
-
批准号:8708236
-
项目类别:
-
资助金额:$24.02万
-
财政年份:2013
-
负责人:Richard P Kraig
-
依托单位:
MULTIPLEXED & CELL-SPECIFIC ANALYSES OF BRAIN FUNCTION
-
批准号:6606561
-
项目类别:
-
资助金额:$17.63万
-
财政年份:2003
-
负责人:Richard P Kraig
-
依托单位:
MULTIPLEXED & CELL-SPECIFIC ANALYSES OF BRAIN FUNCTION
-
批准号:6698827
-
项目类别:
-
资助金额:$17.63万
-
财政年份:2003
-
负责人:Richard P Kraig
-
依托单位:
GLIAL REACTION TO ISCHEMIC BRAIN INJURY
-
批准号:6330411
-
项目类别:
-
资助金额:$28.28万
-
财政年份:1983
-
负责人:Richard P Kraig
-
依托单位:
GLIAL REACTION TO ISCHEMIC BRAIN INJURY
-
批准号:6126094
-
项目类别:
-
资助金额:$27.62万
-
财政年份:1983
-
负责人:Richard P Kraig
-
依托单位:
ASTROGLIAL REACTION TO ISCHEMIC BRAIN INJURY
-
批准号:3399116
-
项目类别:
-
资助金额:$21.29万
-
财政年份:1983
-
负责人:Richard P Kraig
-
依托单位:
ASTROGLIAL REACTION TO ISCHEMIC BRAIN INJURY
-
批准号:2263537
-
项目类别:
-
资助金额:$18.22万
-
财政年份:1983
-
负责人:Richard P Kraig
-
依托单位:
MICROMETABOLISM OF SELECTIVE BRAIN ISCHEMIC INJURY
-
批准号:3078119
-
项目类别:
-
资助金额:$5.7万
-
财政年份:1983
-
负责人:Richard P Kraig
-
依托单位:
GLIAL REACTION TO ISCHEMIC BRAIN INJURY
-
批准号:2767218
-
项目类别:
-
资助金额:$28.61万
-
财政年份:1983
-
负责人:Richard P Kraig
-
依托单位:
GLIAL REACTION TO ISCHEMIC BRAIN INJURY
-
批准号:6477282
-
项目类别:
-
资助金额:$28.96万
-
财政年份:1983
-
负责人:Richard P Kraig
-
依托单位:
Glial Reaction to Ischemic Brain Injury
-
批准号:7874437
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项目类别:
-
资助金额:$36.89万
-
财政年份:1983
-
负责人:Richard P Kraig
-
依托单位:
GLIAL REACTION TO ISCHEMIC BRAIN INJURY
-
批准号:6625543
-
项目类别:
-
资助金额:$29.66万
-
财政年份:1983
-
负责人:Richard P Kraig
-
依托单位:
Glial Reaction to Ischemic Brain Injury
-
批准号:7149563
-
项目类别:
-
资助金额:$38.38万
-
财政年份:1983
-
负责人:Richard P Kraig
-
依托单位:
ASTROGLIAL REACTION TO ISCHEMIC BRAIN INJURY
-
批准号:2263536
-
项目类别:
-
资助金额:$20.11万
-
财政年份:1983
-
负责人:Richard P Kraig
-
依托单位:
HYDROGEN IONS AND BRAIN INFARCTION
-
批准号:3399118
-
项目类别:
-
资助金额:$6.16万
-
财政年份:1983
-
负责人:Richard P Kraig
-
依托单位:
MICROMETABOLISM OF SELECTIVE BRAIN ISCHEMIC INJURY
-
批准号:3078118
-
项目类别:
-
资助金额:$5.73万
-
财政年份:1983
-
负责人:Richard P Kraig
-
依托单位:
国内基金
海外基金
Ascl1介导Wnt/beta-catenin通路在TLE海马硬化中反应性Astrocytes异常增生的作用及调控机制
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批准号:31760279
-
项目类别:地区科学基金项目
-
资助金额:35.0万元
-
批准年份:2017
-
负责人:丁银秀
-
依托单位: