HYPOTHERMIA/ADENOSINE NEUROPROJECTION FOR BIRTH ASPHYXIA

低温/腺苷神经投射治疗出生窒息

• 批准号: 2445638
• 负责人: Judith Noble Halle
• 金额: $ 2.44万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2445638
• 关键词: adenosine; chemoprevention; combination therapy; cryotherapy; disease /disorder prevention /control; embryo /fetus hypoxia; immunocytochemistry; laboratory rat; light microscopy; microdialysis; neuroprotectants; newborn animals; nonhuman therapy evaluation; respiratory distress syndrome of newborn

Hypoxic-ischemic encephalopathy remains the main neurological complication in the newborn. The long range purpose of this investigation is to discover new therapies to prevent brain damage due to birth asphyxia and its devastating sequelae. The specific aims of this study are: (1) To assess the neuroprotective effects of either postischemic mild hypothermia or adenosine potentiation in the neonatal rat pup (2). To assess the combined neuroprotective effects of postischemic mild hypothermia and adenosine potentiation in the neonatal rat pup. It is hypothesized that both hypothermia and Ado agents individually will be neuroprotective and in combination will have an even greater benefit, as measured by comparative hemispheric brain weights, infarct size, alterations in neuronal morphology scores and glutamate levels, using light microscopy, immunohistochemistry and neurochemistry microdialysis as appropriate. The animal model will be 21 litters (10-12 pups/litter) of 7 d old Sprague-Dawley rat pups, exposed to the Rice Vannucci model for hypoxic-ischemic insult and various combinations of recovery conditions with hypothermia and adenosine (Ado) potentiating agents. This research hopes to shed light on a crucial clinical question, regarding the relationship of thermal management, adenosine potentiation and neuroprotection.

缺氧缺血性脑病仍然是主要的神经系统疾病 新生儿的并发症。这次调查的长远目的 是发现新的治疗方法来预防因出生而造成的脑损伤 窒息及其毁灭性后遗症。这项研究的具体目的 目的：(1)评估脑缺血后的神经保护作用。 新生大鼠幼鼠的亚低温或腺苷增强(2)。 评价轻度脑缺血后的联合神经保护作用 新生大鼠仔鼠的低温和腺苷增强作用。它是 假设体温过低和ADO药剂将分别 神经保护和联合使用将有更大的好处，因为 通过比较大脑半球重量，脑梗塞面积， 神经元形态评分和谷氨酸水平的变化，使用 光镜、免疫组织化学和神经化学微透析 视情况而定。动物模型将是21窝(10-12只/窝) 7 d龄Spraogue-Dawley大鼠幼鼠暴露于大米-万努奇模型 用于缺氧缺血性损伤和各种恢复组合 使用低温和腺苷(ADO)增强剂的条件。这 研究希望阐明一个关键的临床问题，关于 热管理、腺苷增强与细胞免疫功能的关系 神经保护。